Microchromosome BAC-FISH Reveals Different Patterns of Genome Organization in Three Charadriiformes Species
Microchromosomes, once considered unimportant elements of the genome, represent fundamental building blocks of bird karyotypes. Shorebirds (Charadriiformes) comprise a wide variety of approximately 390 species and are considered a valuable model group for biological studies. Despite this variety, cy...
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ftdoajarticles:oai:doaj.org/article:254b7deb3e024d1db148369f0171cdc2 2023-05-15T15:48:26+02:00 Microchromosome BAC-FISH Reveals Different Patterns of Genome Organization in Three Charadriiformes Species Marcelo Santos de Souza Suziane Alves Barcellos Michelly da Silva dos Santos Ricardo José Gunski Analía del Valle Garnero Edivaldo Herculano Corrêa de Oliveira Rebecca E. O’Connor Darren K. Griffin Rafael Kretschmer 2022-11-01T00:00:00Z https://doi.org/10.3390/ani12213052 https://doaj.org/article/254b7deb3e024d1db148369f0171cdc2 EN eng MDPI AG https://www.mdpi.com/2076-2615/12/21/3052 https://doaj.org/toc/2076-2615 doi:10.3390/ani12213052 2076-2615 https://doaj.org/article/254b7deb3e024d1db148369f0171cdc2 Animals, Vol 12, Iss 3052, p 3052 (2022) microchromosome avian karyotype bird BAC FISH comparative genomics Veterinary medicine SF600-1100 Zoology QL1-991 article 2022 ftdoajarticles https://doi.org/10.3390/ani12213052 2022-12-30T19:44:14Z Microchromosomes, once considered unimportant elements of the genome, represent fundamental building blocks of bird karyotypes. Shorebirds (Charadriiformes) comprise a wide variety of approximately 390 species and are considered a valuable model group for biological studies. Despite this variety, cytogenetic analysis is still very scarce in this bird order. Thus, the aim of this study was to provide insight into the Charadriiformes karyotype, with emphasis on microchromosome evolution in three species of shorebirds— Calidris canutus , Jacana jacana , and Vanellus chilensis —combining classical and molecular approaches. Cross-species FISH mapping applied two BAC probes for each microchromosome, GGA10–28 (except GGA16). The experiments revealed different patterns of microchromosome organization in the species investigated. Hence, while in C . canutus, we found two microchromosomes involved in chromosome fusions, they were present as single pairs in V . chilensis . We also described a new chromosome number for C . canutus (2n = 92). Hence, this study contributed to the understanding of genome organization and evolution of three shorebird species. Article in Journal/Newspaper Calidris canutus Directory of Open Access Journals: DOAJ Articles Animals 12 21 3052 |
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Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
microchromosome avian karyotype bird BAC FISH comparative genomics Veterinary medicine SF600-1100 Zoology QL1-991 |
spellingShingle |
microchromosome avian karyotype bird BAC FISH comparative genomics Veterinary medicine SF600-1100 Zoology QL1-991 Marcelo Santos de Souza Suziane Alves Barcellos Michelly da Silva dos Santos Ricardo José Gunski Analía del Valle Garnero Edivaldo Herculano Corrêa de Oliveira Rebecca E. O’Connor Darren K. Griffin Rafael Kretschmer Microchromosome BAC-FISH Reveals Different Patterns of Genome Organization in Three Charadriiformes Species |
topic_facet |
microchromosome avian karyotype bird BAC FISH comparative genomics Veterinary medicine SF600-1100 Zoology QL1-991 |
description |
Microchromosomes, once considered unimportant elements of the genome, represent fundamental building blocks of bird karyotypes. Shorebirds (Charadriiformes) comprise a wide variety of approximately 390 species and are considered a valuable model group for biological studies. Despite this variety, cytogenetic analysis is still very scarce in this bird order. Thus, the aim of this study was to provide insight into the Charadriiformes karyotype, with emphasis on microchromosome evolution in three species of shorebirds— Calidris canutus , Jacana jacana , and Vanellus chilensis —combining classical and molecular approaches. Cross-species FISH mapping applied two BAC probes for each microchromosome, GGA10–28 (except GGA16). The experiments revealed different patterns of microchromosome organization in the species investigated. Hence, while in C . canutus, we found two microchromosomes involved in chromosome fusions, they were present as single pairs in V . chilensis . We also described a new chromosome number for C . canutus (2n = 92). Hence, this study contributed to the understanding of genome organization and evolution of three shorebird species. |
format |
Article in Journal/Newspaper |
author |
Marcelo Santos de Souza Suziane Alves Barcellos Michelly da Silva dos Santos Ricardo José Gunski Analía del Valle Garnero Edivaldo Herculano Corrêa de Oliveira Rebecca E. O’Connor Darren K. Griffin Rafael Kretschmer |
author_facet |
Marcelo Santos de Souza Suziane Alves Barcellos Michelly da Silva dos Santos Ricardo José Gunski Analía del Valle Garnero Edivaldo Herculano Corrêa de Oliveira Rebecca E. O’Connor Darren K. Griffin Rafael Kretschmer |
author_sort |
Marcelo Santos de Souza |
title |
Microchromosome BAC-FISH Reveals Different Patterns of Genome Organization in Three Charadriiformes Species |
title_short |
Microchromosome BAC-FISH Reveals Different Patterns of Genome Organization in Three Charadriiformes Species |
title_full |
Microchromosome BAC-FISH Reveals Different Patterns of Genome Organization in Three Charadriiformes Species |
title_fullStr |
Microchromosome BAC-FISH Reveals Different Patterns of Genome Organization in Three Charadriiformes Species |
title_full_unstemmed |
Microchromosome BAC-FISH Reveals Different Patterns of Genome Organization in Three Charadriiformes Species |
title_sort |
microchromosome bac-fish reveals different patterns of genome organization in three charadriiformes species |
publisher |
MDPI AG |
publishDate |
2022 |
url |
https://doi.org/10.3390/ani12213052 https://doaj.org/article/254b7deb3e024d1db148369f0171cdc2 |
genre |
Calidris canutus |
genre_facet |
Calidris canutus |
op_source |
Animals, Vol 12, Iss 3052, p 3052 (2022) |
op_relation |
https://www.mdpi.com/2076-2615/12/21/3052 https://doaj.org/toc/2076-2615 doi:10.3390/ani12213052 2076-2615 https://doaj.org/article/254b7deb3e024d1db148369f0171cdc2 |
op_doi |
https://doi.org/10.3390/ani12213052 |
container_title |
Animals |
container_volume |
12 |
container_issue |
21 |
container_start_page |
3052 |
_version_ |
1766383401275752448 |