Microchromosome BAC-FISH Reveals Different Patterns of Genome Organization in Three Charadriiformes Species

Microchromosomes, once considered unimportant elements of the genome, represent fundamental building blocks of bird karyotypes. Shorebirds (Charadriiformes) comprise a wide variety of approximately 390 species and are considered a valuable model group for biological studies. Despite this variety, cy...

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Bibliographic Details
Published in:Animals
Main Authors: Marcelo Santos de Souza, Suziane Alves Barcellos, Michelly da Silva dos Santos, Ricardo José Gunski, Analía del Valle Garnero, Edivaldo Herculano Corrêa de Oliveira, Rebecca E. O’Connor, Darren K. Griffin, Rafael Kretschmer
Format: Article in Journal/Newspaper
Language:English
Published: MDPI AG 2022
Subjects:
microchromosome
avian karyotype
bird
BAC
FISH
comparative genomics
Veterinary medicine
SF600-1100
Zoology
QL1-991
Calidris canutus
Online Access:https://doi.org/10.3390/ani12213052
https://doaj.org/article/254b7deb3e024d1db148369f0171cdc2
Description
Summary:Microchromosomes, once considered unimportant elements of the genome, represent fundamental building blocks of bird karyotypes. Shorebirds (Charadriiformes) comprise a wide variety of approximately 390 species and are considered a valuable model group for biological studies. Despite this variety, cytogenetic analysis is still very scarce in this bird order. Thus, the aim of this study was to provide insight into the Charadriiformes karyotype, with emphasis on microchromosome evolution in three species of shorebirds— Calidris canutus , Jacana jacana , and Vanellus chilensis —combining classical and molecular approaches. Cross-species FISH mapping applied two BAC probes for each microchromosome, GGA10–28 (except GGA16). The experiments revealed different patterns of microchromosome organization in the species investigated. Hence, while in C . canutus, we found two microchromosomes involved in chromosome fusions, they were present as single pairs in V . chilensis . We also described a new chromosome number for C . canutus (2n = 92). Hence, this study contributed to the understanding of genome organization and evolution of three shorebird species.

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