Comparative study of adenoviruses with monoclonal antibodies Estudo comparativo de diferentes tipos de adenovirus através de anticorpos monoclonais

The obtainment of monoclonal antibodies for adenovirus species 4(Ad4) is described.The specificities of selected monoclonal antibodies were determined by means of viral neutralization test in cell culture, immunofluorescence and Enzyme-Linked Immunosorbent Assay (ELISA), in the presence of the follo...

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Bibliographic Details
Published in:Revista do Instituto de Medicina Tropical de São Paulo
Main Authors: Terezinha Maria de Paiva, Sueko Takimoto, María Akíko Ishida, María Candida Oliveira de Souza, Tuneo Ishimaru, Jorge Neumann, Jorge Kalil
Format: Article in Journal/Newspaper
Language:English
Published: Universidade de São Paulo (USP) 1992
Subjects:
Adenovirus
Species Specific
Monoclonal antibodies
Adenovirus 4
Antigenic Relationship
Arctic medicine. Tropical medicine
RC955-962
Infectious and parasitic diseases
RC109-216
Arctic
Online Access:https://doi.org/10.1590/S0036-46651992000100004
https://doaj.org/article/24df632bb98e42a3a9769639996239af
Description
Summary:The obtainment of monoclonal antibodies for adenovirus species 4(Ad4) is described.The specificities of selected monoclonal antibodies were determined by means of viral neutralization test in cell culture, immunofluorescence and Enzyme-Linked Immunosorbent Assay (ELISA), in the presence of the following species of human adenovirus: 1, 2, 5 (subgenus C), 4 (subgenus E), 7 and 16 (subgenus B) and 9 (subgenus D). Two monoclonal antibodies species specific to adenovirus 4 (1CIII and 3DIII) and one monoclonal antibody that cross reacted with adenovirus species 4 and 7 (2HIII) were obtained. O estudo relata a obtenção de anticorpos monoclonais para o adenovirus tipo (espécie) 4. A especificidade dos anticorpos monoclonais, selecionados nesse experimento, foi determinada testando-os frente às diferentes espécies de adenovírus: 1, 2, 5 (subgênero C), 4 (subgênero E), 7 e 16 (subgênero B) e 9 (subgênero D), pelas técnicas de neutralização em cultura de células, imunofluorescência e ensaio imunoenzimático (ELISA). Os resultados demonstram a obtenção de anticorpos monoclonais que reagiram de maneira específica para o adenovírus 4 (1CIII e 3DIII) e anticorpo monoclonal apresentando reação cruzada com as espécies de adenovírus 4 e 7 (2HIII).

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