Potential TSPO Ligand and Photooxidation Quencher Isorenieratene from Arctic Ocean Rhodococcus sp. B7740

• Published in: Marine Drugs
• Main Authors: Yashu Chen, Mengyao Guo, Jifang Yang, Jigang Chen, Bijun Xie, Zhida Sun
• Format: Article in Journal/Newspaper
• Language: English
• Published: MDPI AG 2019
• Subjects: isorenieratene; macular xanthophylls; TSPO; EPR; antioxidant and anti-UVB radiation; Biology (General); QH301-705.5; Arctic; Arctic Ocean
• Online Access: https://doi.org/10.3390/md17060316; https://doaj.org/article/23fe31531cdf4c43b9283c472f0e2df3

Due to its special aromatic structure, isorenieratene is thought to be an active natural antioxidant and photo/UV damage inhibitor. In this work, isorenieratene that was extracted from Rhodococcus sp. B7740 isolated from the Arctic Ocean, showed excellent scavenging ability of both singlet oxygen and hydroxyl radical in the UVB-induced auto-oxidation process using the EPR method. Within an ARPE-19 cell model damaged by UVB radiation, isorenieratene showed fine protective effects (1.13 ± 0.03 fold) compared with macular xanthophylls (MXs) through upregulating of tspo . The molecular docking was firstly performed to investigate the interaction of isorenieratene with TSPO as a special ligand. Results showed isorenieratene might form a better binding conformation (S-score −8.5438) than MXs and indicate that isorenieratene not only can function as a direct antioxidant but also activate tspo in ARPE-19 cells. Thus, isorenieratene might ease the UV-related damages including age-related macular degeneration (AMD).