Optimization of Preparation Process of Polypeptide Metal Chelates from the Scales of American red fish (Sciaenops ocellatus)
In this paper, the preparation of polypeptides and the synthesis of polypeptide metal chelates from American red fish(Sciaenops ocellatus )scales were studied and optimized. Polypeptides were prepared from the scales of American red fish. The molecular weight of the peptides was analyzed by gel chro...
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ftdoajarticles:oai:doaj.org/article:2309501142424eb7ae2ca774ddf83119 2023-05-15T18:15:24+02:00 Optimization of Preparation Process of Polypeptide Metal Chelates from the Scales of American red fish (Sciaenops ocellatus) Jingliang WAN Peng WU Ling LIN Yuansen LIU Xu TANG Chang’an XU 2022-04-01T00:00:00Z https://doi.org/10.13386/j.issn1002-0306.2021060150 https://doaj.org/article/2309501142424eb7ae2ca774ddf83119 ZH chi The editorial department of Science and Technology of Food Industry http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2021060150 https://doaj.org/toc/1002-0306 1002-0306 doi:10.13386/j.issn1002-0306.2021060150 https://doaj.org/article/2309501142424eb7ae2ca774ddf83119 Shipin gongye ke-ji, Vol 43, Iss 8, Pp 165-171 (2022) fish scales sciaenops ocellatus polypeptide polypeptide metal chelate process optimization Food processing and manufacture TP368-456 article 2022 ftdoajarticles https://doi.org/10.13386/j.issn1002-0306.2021060150 2022-12-30T19:38:29Z In this paper, the preparation of polypeptides and the synthesis of polypeptide metal chelates from American red fish(Sciaenops ocellatus )scales were studied and optimized. Polypeptides were prepared from the scales of American red fish. The molecular weight of the peptides was analyzed by gel chromatography. Polypeptide metal chelates were synthesized by water system method and the metal chelating rate was determined by EDTA complexometric titration method. L9(34) orthogonal test was used to optimize the processing conditions of polypeptide metal chelates. The binding of polypeptide to metal was verified by detecting polypeptide and metal chelate by infrared spectroscopy. The molecular weight of polypeptide was about 1~6 kDa by gel chromatography analysis. The preparation conditions of polypeptide zinc chelate were obtained by orthogonal experiment, that is, the mass ratio of zinc chelate was 2:1, pH5.5, temperature 55℃, time 60 min. The optimal chelating conditions were mass ratio 2:1, pH6.0, temperature 35℃ and time 55 min. Under the optimized condition, the chelate ratio of zinc and copper were 78.21% and 91.24% respectively. The infrared absorption spectra showed that Zn2+, Cu2+ and -NH2 were successfully combined. The results showed that the chelation rate was significantly improved under the condition of this process, and it was rich in metal ions, low cost and promising market prospect, which would be conducive to the high-value application of marine fishery processing by-products and promote the technological upgrading of fishery processing industry. Article in Journal/Newspaper Sciaenops ocellatus Directory of Open Access Journals: DOAJ Articles |
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topic |
fish scales sciaenops ocellatus polypeptide polypeptide metal chelate process optimization Food processing and manufacture TP368-456 |
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fish scales sciaenops ocellatus polypeptide polypeptide metal chelate process optimization Food processing and manufacture TP368-456 Jingliang WAN Peng WU Ling LIN Yuansen LIU Xu TANG Chang’an XU Optimization of Preparation Process of Polypeptide Metal Chelates from the Scales of American red fish (Sciaenops ocellatus) |
topic_facet |
fish scales sciaenops ocellatus polypeptide polypeptide metal chelate process optimization Food processing and manufacture TP368-456 |
description |
In this paper, the preparation of polypeptides and the synthesis of polypeptide metal chelates from American red fish(Sciaenops ocellatus )scales were studied and optimized. Polypeptides were prepared from the scales of American red fish. The molecular weight of the peptides was analyzed by gel chromatography. Polypeptide metal chelates were synthesized by water system method and the metal chelating rate was determined by EDTA complexometric titration method. L9(34) orthogonal test was used to optimize the processing conditions of polypeptide metal chelates. The binding of polypeptide to metal was verified by detecting polypeptide and metal chelate by infrared spectroscopy. The molecular weight of polypeptide was about 1~6 kDa by gel chromatography analysis. The preparation conditions of polypeptide zinc chelate were obtained by orthogonal experiment, that is, the mass ratio of zinc chelate was 2:1, pH5.5, temperature 55℃, time 60 min. The optimal chelating conditions were mass ratio 2:1, pH6.0, temperature 35℃ and time 55 min. Under the optimized condition, the chelate ratio of zinc and copper were 78.21% and 91.24% respectively. The infrared absorption spectra showed that Zn2+, Cu2+ and -NH2 were successfully combined. The results showed that the chelation rate was significantly improved under the condition of this process, and it was rich in metal ions, low cost and promising market prospect, which would be conducive to the high-value application of marine fishery processing by-products and promote the technological upgrading of fishery processing industry. |
format |
Article in Journal/Newspaper |
author |
Jingliang WAN Peng WU Ling LIN Yuansen LIU Xu TANG Chang’an XU |
author_facet |
Jingliang WAN Peng WU Ling LIN Yuansen LIU Xu TANG Chang’an XU |
author_sort |
Jingliang WAN |
title |
Optimization of Preparation Process of Polypeptide Metal Chelates from the Scales of American red fish (Sciaenops ocellatus) |
title_short |
Optimization of Preparation Process of Polypeptide Metal Chelates from the Scales of American red fish (Sciaenops ocellatus) |
title_full |
Optimization of Preparation Process of Polypeptide Metal Chelates from the Scales of American red fish (Sciaenops ocellatus) |
title_fullStr |
Optimization of Preparation Process of Polypeptide Metal Chelates from the Scales of American red fish (Sciaenops ocellatus) |
title_full_unstemmed |
Optimization of Preparation Process of Polypeptide Metal Chelates from the Scales of American red fish (Sciaenops ocellatus) |
title_sort |
optimization of preparation process of polypeptide metal chelates from the scales of american red fish (sciaenops ocellatus) |
publisher |
The editorial department of Science and Technology of Food Industry |
publishDate |
2022 |
url |
https://doi.org/10.13386/j.issn1002-0306.2021060150 https://doaj.org/article/2309501142424eb7ae2ca774ddf83119 |
genre |
Sciaenops ocellatus |
genre_facet |
Sciaenops ocellatus |
op_source |
Shipin gongye ke-ji, Vol 43, Iss 8, Pp 165-171 (2022) |
op_relation |
http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2021060150 https://doaj.org/toc/1002-0306 1002-0306 doi:10.13386/j.issn1002-0306.2021060150 https://doaj.org/article/2309501142424eb7ae2ca774ddf83119 |
op_doi |
https://doi.org/10.13386/j.issn1002-0306.2021060150 |
_version_ |
1766188492550832128 |