Genomics- and Transcriptomics-Guided Discovery of Clavatols from Arctic Fungi Penicillium sp. MYA5
Clavatols exhibit a wide range of biological activities due to their diverse structures. A genome mining strategy identified an A5cla cluster from Penicillium sp. MYA5, derived from the Arctic plant Dryas octopetala , is responsible for clavatol biosynthesis. Seven clavatols, including one new clava...
| Published in: | Marine Drugs |
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| Main Authors: | , , , , , , , , |
| Format: | Article in Journal/Newspaper |
| Language: | English |
| Published: |
MDPI AG
2024
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| Subjects: | |
| Online Access: | https://doi.org/10.3390/md22060236 https://doaj.org/article/229a1288064549de85fcdf48bf5e3604 |
| Summary: | Clavatols exhibit a wide range of biological activities due to their diverse structures. A genome mining strategy identified an A5cla cluster from Penicillium sp. MYA5, derived from the Arctic plant Dryas octopetala , is responsible for clavatol biosynthesis. Seven clavatols, including one new clavatol derivate named penicophenone F ( 1 ) and six known clavatols ( 2 – 7 ), were isolated from Penicillium sp. MYA5 using a transcriptome mining strategy. These structures were elucidated by comprehensive spectroscopic analysis. Antibacterial, aldose reductase inhibition, and siderophore-producing ability assays were conducted on compounds 1 – 7 . Compounds 1 and 2 demonstrated inhibitory effects on the ALR2 enzyme with inhibition rates of 75.3% and 71.6% at a concentration of 10 μM, respectively. Compound 6 exhibited antibacterial activity against Staphylococcus aureus and Escherichia coli with MIC values of 4.0 μg/mL and 4.0 μg/mL, respectively. Additionally, compounds 1 , 5 , and 6 also showed potential iron-binding ability. |
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