Endolithic Fungal Diversity in Antarctic Oligocene Rock Samples Explored Using DNA Metabarcoding

In this study, we evaluated the fungal diversity present associated with cores of Oligocene rocks using a DNA metabarcoding approach. We detected 940,969 DNA reads grouped into 198 amplicon sequence variants (ASVs) representing the phyla Ascomycota , Basidiomycota , Mortierellomycota , Chytridiomyco...

Full description

Bibliographic Details
Published in:Biology
Main Authors: Natana G. Rabelo, Vívian N. Gonçalves, Marcelo A. Carvalho, Sandro M. Scheffler, Gustavo Santiago, Paula A. Sucerquia, Fabio S. Oliveira, Larissa P. Campos, Fabyano A. C. Lopes, Karita C. R. Santos, Micheline C. Silva, Peter Convey, Paulo E. A. S. Câmara, Luiz H. Rosa
Format: Article in Journal/Newspaper
Language:English
Published: MDPI AG 2024
Subjects:
Antarctica
eDNA
fungi
environmental drivers
Biology (General)
QH301-705.5
Antarc*
Antarctic
Online Access:https://doi.org/10.3390/biology13060414
https://doaj.org/article/20895502b0e64f6f9e7365e571f3b3c9
Description
Summary:In this study, we evaluated the fungal diversity present associated with cores of Oligocene rocks using a DNA metabarcoding approach. We detected 940,969 DNA reads grouped into 198 amplicon sequence variants (ASVs) representing the phyla Ascomycota , Basidiomycota , Mortierellomycota , Chytridiomycota , Mucoromycota , Rozellomycota , Blastocladiomycota , Monoblepharomycota , Zoopagomycota , Aphelidiomycota (Fungi) and the fungal-like Oomycota (Stramenopila), in rank abundance order. Pseudogymnoascus pannorum , Penicillium sp., Aspergillus sp., Cladosporium sp., Aspergillaceae sp. and Diaporthaceae sp. were assessed to be dominant taxa, with 22 fungal ASVs displaying intermediate abundance and 170 being minor components of the assigned fungal diversity. The data obtained displayed high diversity indices, while rarefaction indicated that the majority of the diversity was detected. However, the diversity indices varied between the cores analysed. The endolithic fungal community detected using a metabarcoding approach in the Oligocene rock samples examined contains a rich and complex mycobiome comprising taxa with different lifestyles, comparable with the diversity reported in recent studies of a range of Antarctic habitats. Due to the high fungal diversity detected, our results suggest the necessity of further research to develop strategies to isolate these fungi in culture for evolutionary, physiological, and biogeochemical studies, and to assess their potential role in biotechnological applications.

Similar Items