Derivation of trypsin-like enzymes from Antarctic marine organisms
Modern biotechnology provides continuous search for alternative sources of raw materials. The aim of this work was to isolate and characterize trypsin-like enzymes from tissues of Antarctic marine aquatic organisms (krill, starfish, nemertines). Trypsin fraction was obtained by column chromatography...
| Main Authors: | , , , |
|---|---|
| Format: | Article in Journal/Newspaper |
| Language: | English Ukrainian |
| Published: |
Taras Shevchenko National University of Kyiv
2016
|
| Subjects: | |
| Online Access: | https://doaj.org/article/1e75b4b0744442d88fd046622d9ae8b4 |
| Summary: | Modern biotechnology provides continuous search for alternative sources of raw materials. The aim of this work was to isolate and characterize trypsin-like enzymes from tissues of Antarctic marine aquatic organisms (krill, starfish, nemertines). Trypsin fraction was obtained by column chromatography on benzamidine-Sepharose. Proteolytic activity in the resulting fraction was revealed. Analysis of protein fractions was performed by disc-electrophoresis in a 10% polyacrylamide gel. The presence of active hydrolases with different molecular weights in the tissues of aquatic organisms was demonstrated. Protein bands with molecular weights below 10-14 kDa may be trypsin-like enzyme fragments that were subjected to autolysis process. |
|---|