INFLUENCE OF METFORMIN AND ITS COMBINATION WITH SODIUM HYDROGEN SULPHIDE ON H2 S SYSTEM AND ASSOCIATED BIOCHEMICAL DISORDERS IN MYOCARDIUM AND KIDNEY OF RATS WITH STREPTOZOTOCININDUCED DIABETES
Diabetes mellitus (DM) is a recent problem due to the high prevalence of the disease and the high mortality of patients. The most common complications of type I and II diabetes include heart and kidney damage. Metformin, as a first-line drug in the treatment of type 2 diabetes, are known to reduce t...
| Published in: | Bulletin of Problems Biology and Medicine |
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| Main Authors: | , , , |
| Format: | Article in Journal/Newspaper |
| Language: | English Russian Ukrainian |
| Published: |
Ukrainian Medical Stomatological Academy
2020
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| Subjects: | |
| Online Access: | https://doi.org/10.29254/2077-4214-2020-3-157-133-137 https://doaj.org/article/1da4c455db574a10b186d23e0636fa4c |
| Summary: | Diabetes mellitus (DM) is a recent problem due to the high prevalence of the disease and the high mortality of patients. The most common complications of type I and II diabetes include heart and kidney damage. Metformin, as a first-line drug in the treatment of type 2 diabetes, are known to reduce the risk of cardiovascular and renal complications. Recently, metformin has been shown to increase H2 S in the organs of rats, which is a known cardio- and nephroprotector. However, the effect of metformin on H2 S metabolism in the heart and kidneys in experimental diabetes remains uncertain. The ability of H2 S donors to modify the antidiabetic effect of metformin is also unknown. Objective: to evaluate the effect of metformin and its combination with sodium hydrogen sulfide on H2 S system, the content of profibrogenic mediator galectin-3, the activity of free radical oxidation of lipids and proteins in the heart and kidneys of rats with streptozotocin-induced diabetes. Object and methods of research. The experiments were carried out on 40 white laboratory rats of both sexes, weight 220-280 g. DM model was initiated by a single intraperitoneal injection of streptozotocin (40 mg/kg) in three groups of animals. From the 3rd to the 28th day after streptozotocin injection, one group of animals was administered metformin (500 mg/kg) intragastrically once per day, and the other group along with metformin was administered a H2 S donor – NaHS • H2 O (3 mg/kg) once per day intraperitoneally. Peripheral blood glucose was measured using a glucometer, galectin-3 levels were determined by enzyme-linked immunosorbent assay (ELISA) kit in myocardial and renal homogenates, H2 S content, cystathionine-γ-lyase (CGL) activity, levels of malonic dialdehyde (MDA group) and carbonic acid. Statistical processing of the results was performed using SPSS Statistica 17.0. Research results. According to our results, a single injection of streptozotocin caused a number of metabolic disturbances : an increase in blood glucose by 4.6 times (p ... |
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