Bioinspired Lipase Immobilized Membrane for Improving Hesperidin Lipophilization

Lipophilization is a promising way to improve the bioavailability of flavonoids. However, the traditional enzymatic esterification methods are time-consuming, and present low yields and purity. Herein, a novel membrane-based lipophilization technology—bioinspired lipase immobilized membranes (BLIMs)...

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Bibliographic Details
Published in:Antioxidants
Main Authors: Shanxiu Ming, Shuyi Li, Zhe Chen, Xujun Chen, Feifei Wang, Shaonan Deng, Krystian Marszałek, Zhenzhou Zhu, Wenxiang Zhang, Francisco J. Barba
Format: Article in Journal/Newspaper
Language:English
Published: MDPI AG 2022
Subjects:
Candida antarctica lipase B
enzymatic esterification
membrane separation
hesperidin lipophilization
bioinspired lipase immobilized membrane
Therapeutics. Pharmacology
RM1-950
Antarc*
Antarctica
Online Access:https://doi.org/10.3390/antiox11101906
https://doaj.org/article/1c9c4b0227984fc5a1cdfc891b23c6cc
Description
Summary:Lipophilization is a promising way to improve the bioavailability of flavonoids. However, the traditional enzymatic esterification methods are time-consuming, and present low yields and purity. Herein, a novel membrane-based lipophilization technology—bioinspired lipase immobilized membranes (BLIMs), including CAL-B@PES, CAL-B@PDA/PES and GA/CAL-B@PDA/PES— were fabricated to improve the antioxidant flavanone glycoside hesperidin lipophilization. Via reverse filtration, PDA coating and GA crosslinking, Candida antarctica lipase B (CAL-B) was stably immobilized on membrane to fabricate BLIMs. Among the three BLIMs, GA/CAL-B@PDA/PES had the greatest enzyme activity and enzyme loading, the strongest tolerance of changes in external environmental conditions (temperatures, pH, heating time, storage time and numbers of cycles) and the highest hesperidin esterification efficiency. Moreover, the optimal operating condition for GA/CAL-B@PDA/PES fabrication was the CAL-B concentration of 0.36 mg/mL, operation pressure of 2 bar, GA concentration of 5% and crosslinking time of 1 h. Afterwards, the hesperidin esterification process did not affect the micromorphology of BLIM, but clearly improved the BLIM permeability and esterified product efficiency. The present study reveals the fabrication mechanism of BLIMs and offers insights into the optimizing strategy that governs the membrane-based lipophilization technology process.

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