Fructose 1,6-diphosphate alleviates myocardial ischemia reperfusion injury in rats through JAK2/STAT3 pathway

• Published in: Asian Pacific Journal of Tropical Medicine
• Main Authors: Ju-Fei Wang, Cheng Jiang
• Format: Article in Journal/Newspaper
• Language: English
• Published: Wolters Kluwer Medknow Publications 2018
• Subjects: fructose 1; 6-diphosphate; myocardial ischemia reperfusion; apoptosis; janus kinase 2; signal transducer; activator of transcription 3; Arctic medicine. Tropical medicine; RC955-962; Arctic; Janus
• Online Access: https://doi.org/10.4103/1995-7645.225023; https://doaj.org/article/19fcc536d6244fa59049f06c694393b9

Objective: To study the effect of fructose 1,6-diphosphate (FDP) on myocardial ischemia reperfusion injury in rats and its molecular mechanism. Methods: Male SPF SD rats were selected as experimental animals and randomly divided into four groups. Sham group received sham operation, I/R group were made into myocardial ischemia reperfusion injury models, FDP group were made into myocardial ischemia reperfusion injury models and then were given FDP intervention, and FDP+AG490 group were made into myocardial ischemia reperfusion injury models and then were given FDP and JAK2 inhibitor AG490 intervention. Results: CK, CK-MB, cTnI and LDH contents in serum as well as Bax and Caspase-3 protein expression in myocardial tissue of I/R group were significantly higher than those of Sham group whereas Bcl-2, p-JAK2 and p-STAT3 protein expression in myocardial tissues were significantly lower than those of Sham group; CK, CK-MB, cTnI and LDH contents in serum as well as Bax and Caspase-3 protein expression in myocardial tissue of FDP group were significantly lower than those of I/R group whereas Bcl-2, p-JAK2 and p-STAT3 protein expression in myocardial tissue were significantly higher than those of I/R group; CK, CK-MB, cTnI and LDH contents in serum as well as Bax and Caspase-3 protein expression in myocardial tissue of FDP+AG490 group were significantly higher than those of FDP group whereas Bcl-2 protein expression in myocardial tissue was significantly lower than that of FDP group. Conclusion: FDP could reduce the myocardial ischemia reperfusion injury in rats by activating the JAK2/STAT3 pathway.