Improved negative selection protocol for Plasmodium berghei in the rodent malarial model

Abstract An improved methodology is presented here for transgenic Plasmodium berghei lines that express the negative selectable marker yFCU (a bifunctional protein that combines yeast cytosine deaminase and uridyl phosphoribosyl transferase (UPRT)) and substitutes delivery of selection drug 5-fluoro...

Full description

Bibliographic Details
Published in:Malaria Journal
Main Authors: Orr Rachael Y, Philip Nisha, Waters Andrew P
Format: Article in Journal/Newspaper
Language:English
Published: BMC 2012
Subjects:
Plasmodium berghei
Negative selection
Transfection
Selectable marker
Malaria
Arctic medicine. Tropical medicine
RC955-962
Infectious and parasitic diseases
RC109-216
Arctic
Online Access:https://doi.org/10.1186/1475-2875-11-103
https://doaj.org/article/16a882e282ed4302b9d628feb937b001
Description
Summary:Abstract An improved methodology is presented here for transgenic Plasmodium berghei lines that express the negative selectable marker yFCU (a bifunctional protein that combines yeast cytosine deaminase and uridyl phosphoribosyl transferase (UPRT)) and substitutes delivery of selection drug 5-fluorocytosine (5FC) by intraperitoneal injection for administration via the drinking water of the mice. The improved methodology is shown to be as effective, less labour-intensive, reduces animal handling and animal numbers required for successful selection thereby contributing to two of the "three Rs" of animal experimentation, namely refinement and reduction.

Similar Items