Comparison between immunomagnetic separation, coupled with immunofluorescence, and the techniques of Faust et al. and of Lutz for the diagnosis of Giardia lamblia cysts in human feces

• Main Authors: Souza Doris Sobral Marques, Barreiros Juliana Teixeira, Papp Karina Maria, Steindel Mário, Simões Cláudia Maria Oliveira, Barardi Célia Regina Monte
• Format: Article in Journal/Newspaper
• Language: English
• Published: Universidade de São Paulo (USP) 2003
• Subjects: Giardia lamblia; Giardiasis; Cryptosporidium; Immunomagnetic separation technique; Diagnosis; Arctic medicine. Tropical medicine; RC955-962; Infectious and parasitic diseases; RC109-216; Arctic
• Online Access: https://doaj.org/article/15e4765a58164cb98d93d94645907ef8

In the present study, the performance of Immunomagnetic Separation technique, coupled with Immunofluorescence (IMS-IFA), was compared with the FAUST et al. and Lutz parasitological techniques for the detection of Giardia lamblia cysts in human feces. One hundred and twenty-seven samples were evaluated by the three techniques at the same time showing a rate of cyst detection of 27.5% by IMS-IFA and 15.7% by both Faust et al. and Lutz techniques. Data analysis showed a higher sensitivity of IMS-IFA for the detection of G. lamblia cysts in comparison with the techniques of FAUST et al. and Lutz. The use of this methodology as a routine procedure enables the processing of many samples simultaneously, in order to increase recovery rate of G. lamblia cysts and reduce the time of sample storage.