Genome-Wide Identification and Expression Profiling of the COMMD Gene Family in Four Bivalve Molluscs

The COMMD (copper metabolism gene MuRR1 domain) gene family, highly conserved among multicellular eukaryotic organisms, plays important roles in a variety of biological processes, ranging from copper homeostasis, ionic transport, protein trafficking, NF-κB-mediated transcription, and cell proliferat...

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Bibliographic Details
Published in:Frontiers in Marine Science
Main Authors: Xiaomei Chen, Naina Hu, Shanshan Lian, Luoan Li, Fengzhi Sun, Lingling Zhang, Shi Wang, Zhenmin Bao, Jingjie Hu
Format: Article in Journal/Newspaper
Language:English
Published: Frontiers Media S.A. 2022
Subjects:
bivalve
COMMD
expression profiling
phylogenetic analysis
innate immune
Science
Q
General. Including nature conservation
geographical distribution
QH1-199.5
Pacific
Crassostrea gigas
Pacific oyster
Online Access:https://doi.org/10.3389/fmars.2022.884991
https://doaj.org/article/0ee005bff1074df6a08858057587df8c
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Summary:The COMMD (copper metabolism gene MuRR1 domain) gene family, highly conserved among multicellular eukaryotic organisms, plays important roles in a variety of biological processes, ranging from copper homeostasis, ionic transport, protein trafficking, NF-κB-mediated transcription, and cell proliferation. However, systematic identification, spatiotemporal expression, and stress-responsive patterns of COMMD genes remain obscure in molluscs. Here, we analyzed the characteristics of the COMMD gene family in four bivalve molluscs based on both genome and extensive transcriptomic resources. Firstly, we investigated the genomic signatures, functional domains, and phylogenetic relationships, and ten single-copy members were identified in Yesso scallop (Patinopecten yessoensis), Zhikong scallop (Chlamys farreri), Pacific oyster (Crassostrea gigas), and dwarf surf clam (Mulinia lateralis), respectively. Strong purifying selection was revealed for COMMD4. Higher expressions of most COMMDs were observed in the hepatopancreas, besides which a different tissue preference of COMMDs’ expression was found among four bivalves. Moreover, in the dwarf surf clam, the responses of COMMD members under stresses were found more sensitive in the hepatopancreas than in the gill, and MlCOMMD9 and MlCOMMD4 might be the good candidate stress indicator genes respectively for copper ion stress and V. Anguillarum infection. Our study would contribute to a better understanding for the evolution of the COMMD gene family and provide valuable information for their innate immune roles in bivalve molluscs.

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