Antifungal potential of punicalagin against Cryptococcus neoformans species complex

ABSTRACT This study evaluated the antifungal activity and cytotoxicity profile of the ellagitannin punicalagin, a compound extracted from the L. pacari A. St.-Hil (Lythraceae) leaf, against Cryptococcus neoformans species complex. Minimum inhibitory concentrations (MIC) were checked using the broth...

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Bibliographic Details
Published in:Revista do Instituto de Medicina Tropical de São Paulo
Main Authors: Thaísa Cristina Silva, Ana Laura de Sene Amâncio Zara, Fabyola Amaral da Silva Sá, Maria Teresa Freitas Bara, Renato Ivan de Ávila, Carolina Rodrigues Costa, Marize Campos Valadares, Andressa Santana dos Santos, Vivianny Aparecida Queiroz Freitas, Maria do Rosário Rodrigues Silva
Format: Article in Journal/Newspaper
Language:English
Published: Universidade de São Paulo (USP) 2018
Subjects:
Punicalagin
Lafoensia pacari A. St.-Hil
Cytotoxicity
Antifungal activity
Cryptococcus neoformans species Complex
Arctic medicine. Tropical medicine
RC955-962
Infectious and parasitic diseases
RC109-216
Arctic
Online Access:https://doi.org/10.1590/s1678-9946201860060
https://doaj.org/article/0e2935f896344d1dbf5690443ea853fd
Description
Summary:ABSTRACT This study evaluated the antifungal activity and cytotoxicity profile of the ellagitannin punicalagin, a compound extracted from the L. pacari A. St.-Hil (Lythraceae) leaf, against Cryptococcus neoformans species complex. Minimum inhibitory concentrations (MIC) were checked using the broth microdilution method. Minimum fungicidal concentrations (MFC) and time of death were used to confirm the antifungal activity of the compound. The in vitro cytotoxicity of punicalagin was tested in BALB/c3T3 fibroblasts and A549 human lung cancer cell line, while the hemolytic potential was tested on sheep erythrocytes. The morphological changes induced in yeast strains by the presence of punicalagin were also analyzed. Tested on eight isolates of the C. neoformans complex punicalagin showed MIC of 0.5 to 4.0 μg/mL and MFC> 256 μg/mL. Punicalagin also demonstrated a good growth inhibitory activity in time-kill curves, but it was not able to achieve a statistically significant reduction of fungal growth suggesting a fungistatic effect of the compound. In vitro cytotoxicity studies using the two cell lines showed that punicalagin has low activity on these cells and no activity on sheep erythrocytes. Morphological changes were seen in the yeasts strains studied when treated with punicalagin. Therefore, punicalagin is a potential antifungal for important pathogenic yeasts and presents a low cytotoxicity profile associated with no hemolytic effects.

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