Effects of ice-binding protein from Leucosporidium on the cryopreservation of boar sperm

The aim of this study was performed to evaluate the effects of ice-binding protein from the arctic yeast Leucosporidium (LeIBP) supplementation on cryopreservation of boar sperm. The collected semen was diluted (1.5×108/ml) in lactose egg yolk (LEY) and cooled at 5°C for 3 h. The cooled semen was th...

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Bibliographic Details
Published in:Journal of Animal Reproduction and Biotechnology
Main Authors: Sang Hyoun Park, Keon Bong Oh, Sun-A Ock, Sung June Byun, Hwi-Cheul Lee, Suresh Kumar, Sung Gu Lee, Jae-Seok Woo
Format: Article in Journal/Newspaper
Language:English
Korean
Published: The Korean Society of Animal Reproduction and Biotechnology 2018
Subjects:
antifreeze protein
boar spermatozoa
cryopreservation
leucosporidium ice-binding protein
lipid peroxidation
reactive oxygen species
Biotechnology
TP248.13-248.65
Medicine (General)
R5-920
Internal medicine
RC31-1245
Arctic
Online Access:https://doi.org/10.12750/JET.2018.33.3.185
https://doaj.org/article/0dc9895dac924a01862fbae4f901dfae
Description
Summary:The aim of this study was performed to evaluate the effects of ice-binding protein from the arctic yeast Leucosporidium (LeIBP) supplementation on cryopreservation of boar sperm. The collected semen was diluted (1.5×108/ml) in lactose egg yolk (LEY) and cooled at 5°C for 3 h. The cooled semen was then diluted (1×108/ml) in LeIBP containing LEY with 9% glycerol and maintained at 5°C for 30 min. The semen was divided into six experimental groups (control, 0.001, 0.005, 0.01, 0.05 and 0.1 mg/ml of LeIBP). The straws were kept on above the liquid nitrogen (LN2) vapors for 20 minutes and then plunged into LN2. After thawing, computer-assisted sperm analysis was used for sperm motility and flow cytometry was performed to assess the viability, acrosome integrity (FITC-PSA/PI), ROS (DCF/PI), lipid peroxidation (BODIPY C11/PI) and apoptosis (Annexin V/PI), respectively. No significant responses were observed for sperm motility. However, sperm viability was significantly increased on 0.05 and 0.1 mg/ml of LeIBP groups compared to control (P < 0.05). In addition, acrosome integrity was significantly increases LeIBP groups (P < 0.05) and both ROS and lipid peroxidation level were lower in all LeIBP groups than those of control (P < 0.05). On the other hand, a significant higher apoptosis rate was observed in 0.05 and 0.1 mg/ml of LeIBP groups compared to control (P < 0.05). It can be assumed that a supplementation of LeIBP in boar sperm freezing extender is an effective method to increase the sperm qualities after cryopreservation.

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