Biodemes and zymodemes of Trypanosoma cruzi strains: correlations with clinical data and experimental pathology
With the objective of establishing biological and biochemical characteristics of a significant number of Trypanosoma cruzi strains from different geographical areas, 138 strains isolated from naturally infected humans, triatomine or vertebrate hosts were studied; 120 were isolated from different are...
| Published in: | Revista da Sociedade Brasileira de Medicina Tropical |
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| Main Authors: | , |
| Format: | Article in Journal/Newspaper |
| Language: | English |
| Published: |
Sociedade Brasileira de Medicina Tropical (SBMT)
1997
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| Subjects: | |
| Online Access: | https://doi.org/10.1590/S0037-86821997000100006 https://doaj.org/article/0d01901bc2ae409aafc7b6d159d92bb2 |
| Summary: | With the objective of establishing biological and biochemical characteristics of a significant number of Trypanosoma cruzi strains from different geographical areas, 138 strains isolated from naturally infected humans, triatomine or vertebrate hosts were studied; 120 were isolated from different areas of Brazil and 18 from other South and Central American countries. Inocula from triatomine or culture forms were injected into suckling Swiss mice, followed by passages into mice 10 to 12 g. Biological characters and histopathological study permitted the inclusion of the strains into three Types or biodemes: I, II, III. Isoenzymic analysis confirmed a correspondence between the biodemes and zymodemes : Type I and Z2b, Type II and Z2, Type III and Z1. Results showed the ubiquitary distribution of the several types of strains. The predominance of the same Type and zymodeme in one geographical area was confirmed : Type II strains among the human cases from eastern Bahia and east of Goiás; Type III strains from humans of north Brazil and Central America and from silvatic vectors or vertebrates from other geographical areas. The biological types of strains correlate with different histopathological lesions considering cardiac involvement and neuronal lesions. These findings suggest that the biological behavior together with isoenzymes patterns and pathological pictures in the vertebrate host can be an important tool for establishing correlations between strains behavior and clinico-pathological manifestations of Chagas' disease in different geographical areas. Foram estudados os caracteres biológicos e isoenzimáticos de 138 cepas do Trypanosoma cruzi de diferentes áreas geográficas, sendo 120 do Brasil e 18 de outros paises da América do Sul e Central. Camundongos recém-nascidos foram inoculados com formas metacíclicas de triatomineos ou de culturas axenicas, seguindo-se passagem em camundongo de 10 a 12g. Os caracteres biológicos e o estudo histopatológico permitiram incluir todas a cepas em três Tipos ou biodemas: I, ... |
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