NEW BIOTECHNOLOGICAL METHODS FOR CRYOPRESERVATION OF REPRODUCTIVE CELLS OF STURGEON

The aim of the research is to increase the survivability of reproductive cells of sturgeon at cryopreservation and developing reliable technology suitable for use on an industrial scale.Methods. We have used standard methods of freezing, thawing reproductive cells, fertilization and incubation of eg...

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Bibliographic Details
Published in:South of Russia: ecology, development
Main Authors: E. N. Ponomareva, A. A. Krasilnikova, A. M. Tikhomirov, A. V. Firsova
Format: Article in Journal/Newspaper
Language:Russian
Published: Kamerton 2016
Subjects:
криоконсервация
сперма рыб
яйцеклетки
криопротектор
осетровые рыбы
выживаемость
сохранение генофонда
искусственное воспроизводство
Ecology
QH540-549.5
Beluga
Beluga*
Online Access:https://doi.org/10.18470/1992-1098-2016-1-59-68
https://doaj.org/article/0c689c377a384a98a0ca2546338ec681
Description
Summary:The aim of the research is to increase the survivability of reproductive cells of sturgeon at cryopreservation and developing reliable technology suitable for use on an industrial scale.Methods. We have used standard methods of freezing, thawing reproductive cells, fertilization and incubation of eggs and larval rearing of sturgeon. Fundamentally new is cryoprotective composition: for sperm we have adjusted the composition of cryoprotective medium (for beluga 3% of dimethyl sulfoxide, for Russian sturgeon 4% of dimethyl sulfoxide); for freezing the eggs we have used cryoprotective mixture of unrefined vegetable and animal oils.Results. Survivability of defrosted sperm sturgeon has been increased: for Beluga it is up to 20%, for Russian sturgeon - 47%. At insemination of cryopreserved eggs of Russian sturgeon with native sperm the fertilization rate has made 41%.Main conclusions. The research proves the effectiveness of reducing the toxic effect of cryoprotective substances, thus leading to increased survivability of reproductive cells of sturgeon. During the insemination of eggs, stored in liquid nitrogen, the resulting offspring were viable and by the reactivity of the central nervous system and receptor complex it does not differ from the young obtained by conventional technology.

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