Linear Aminolipids with Moderate Antimicrobial Activity from the Antarctic Gram-Negative Bacterium Aequorivita sp.

The combination of LC-MS/MS based metabolomics approach and anti-MRSA activity-guided fractionation scheme was applied on the Gram-negative bacterium Aequorivita sp. isolated from shallow Antarctic sea sediment using a miniaturized culture chip technique. This methodology afforded the isolation of t...

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Bibliographic Details
Published in:Marine Drugs
Main Authors: Giuseppina Chianese, Fortunato Palma Esposito, Delphine Parrot, Colin Ingham, Donatella de Pascale, Deniz Tasdemir
Format: Article in Journal/Newspaper
Language:English
Published: MDPI AG 2018
Subjects:
Aequorivita
Gram-negative bacterium
miniaturized culture chip
linear aminolipid
MRSA
LC-MS/MS
Biology (General)
QH301-705.5
Antarctic
The Antarctic
Antarc*
Online Access:https://doi.org/10.3390/md16060187
https://doaj.org/article/0c160fb7c5d449beaf09cadc184e4712
Description
Summary:The combination of LC-MS/MS based metabolomics approach and anti-MRSA activity-guided fractionation scheme was applied on the Gram-negative bacterium Aequorivita sp. isolated from shallow Antarctic sea sediment using a miniaturized culture chip technique. This methodology afforded the isolation of three new (1–3) and four known (4–7) N-terminal glycine- or serine-bearing iso-fatty acid amides esterified with another iso-fatty acid through their C-3 hydroxy groups. The chemical structures of the new compounds were elucidated using a set of spectroscopic (NMR, [α]D and FT-IR) and spectrometric (HRMS, HRMS/MS) methods. The aminolipids possessing an N-terminal glycine unit (1, 2, 4, 5) showed moderate in vitro antimicrobial activity against MRSA (IC50 values 22–145 μg/mL). This is the first in-depth chemistry and biological activity study performed on the microbial genus Aequorivita.

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