Optimization of the Production of Enzymatic Biodiesel from Residual Babassu Oil ( Orbignya sp.) via RSM

Residual oil from babassu ( Orbignya sp.), a low-cost raw material, was used in the enzymatic esterification for biodiesel production, using lipase B from Candida antarctica (Novozym ® 435) and ethanol. For the first time in the literature, residual babassu oil and Novozym ® 435 are being investigat...

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Bibliographic Details
Published in:Catalysts
Main Authors: Katerine S. Moreira, Lourembergue S. Moura Júnior, Rodolpho R. C. Monteiro, André L. B. de Oliveira, Camila P. Valle, Tiago M. Freire, Pierre B. A. Fechine, Maria C. M. de Souza, Gloria Fernandez-Lorente, José M. Guisan, José C. S. dos Santos
Format: Article in Journal/Newspaper
Language:English
Published: MDPI AG 2020
Subjects:
biodiesel
Novozym ® 435
residual oil from babassu
esterification
response surface methodology
Chemical technology
TP1-1185
Chemistry
QD1-999
Antarc*
Antarctica
Online Access:https://doi.org/10.3390/catal10040414
https://doaj.org/article/0beb565cea0d4e8c99d6b19754b3b9ad
Description
Summary:Residual oil from babassu ( Orbignya sp.), a low-cost raw material, was used in the enzymatic esterification for biodiesel production, using lipase B from Candida antarctica (Novozym ® 435) and ethanol. For the first time in the literature, residual babassu oil and Novozym ® 435 are being investigated to obtain biodiesel. In this communication, response surface methodology (RSM) and a central composite design (CCD) were used to optimize the esterification and study the effects of four factors (molar ratio (1:1–1:16, free fatty acids (FFAs) /alcohol), temperature (30–50 °C), biocatalyst content (0.05–0.15 g) and reaction time (2–6 h)) in the conversion into fatty acid ethyl esters. Under optimized conditions (1:18 molar ratio (FFAs/alcohol), 0.14 g of Novozym ® 435, 48 °C and 4 h), the conversion into ethyl esters was 96.8%. It was found that after 10 consecutive cycles of esterification under optimal conditions, Novozym ® 435 showed a maximum loss of activity of 5.8%, suggesting a very small change in the support/enzyme ratio proved by Fourier Transform Infrared (FTIR) spectroscopy and insignificant changes in the surface of Novozym ® 435 proved by scanning electron microscopy (SEM) after the 10 consecutive cycles of esterification.

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