Candida albicans PROTEIN PROFILE CHANGES IN RESPONSE TO THE BUTANOLIC EXTRACT OF Sapindus saponariaL.

Candida albicans is an opportunistic human pathogen that is capable of causing superficial and systemic infections in immunocompromised patients. Extracts of Sapindus saponaria have been used as antimicrobial agents against various organisms. In the present study, we used a combination of two-dimens...

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Bibliographic Details
Published in:Revista do Instituto de Medicina Tropical de São Paulo
Main Authors: Adriana FIORINI, Fabio Rogério ROSADO, Eliane Martins da Silva BETTEGA, Kátia Cristina Sibin MELO, Caroline KUKOLJ, Patrícia de Souza BONFIM-MENDONÇA, Cristiane Suemi SHINOBU-MESQUITA, Luciana Dias GHIRALDI, Paula Aline Zanetti CAMPANERUT, Isis Regina Grenier CAPOCI, Janine Silva Ribeiro GODOY, Izabel Cristina Piloto FERREIRA, Terezinha Inez Estivalet SVIDZINSKI
Format: Article in Journal/Newspaper
Language:English
Published: Universidade de São Paulo (USP) 2016
Subjects:
Candida albicans
Sapindus saponaria
Two-dimensional gel electrophoresis
Saponins
Mass spectrometry
Arctic medicine. Tropical medicine
RC955-962
Infectious and parasitic diseases
RC109-216
Arctic
Online Access:https://doi.org/10.1590/S1678-9946201658025
https://doaj.org/article/0a147989c41b4058a7f419e8313dcb64
Description
Summary:Candida albicans is an opportunistic human pathogen that is capable of causing superficial and systemic infections in immunocompromised patients. Extracts of Sapindus saponaria have been used as antimicrobial agents against various organisms. In the present study, we used a combination of two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) and matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) to identify the changes in protein abundance of C. albicans after exposure to the minimal inhibitory concentration (MIC) and sub-minimal inhibitory concentration (sub-MIC) of the butanolic extract (BUTE) of S. saponaria and also to fluconazole. A total of six different proteins with greater than 1.5 fold induction or repression relative to the untreated control cells were identified among the three treatments. In general, proteins/enzymes involved with the glycolysis (GPM1, ENO1, FBA1), amino acid metabolism (ILV5, PDC11) and protein synthesis (ASC1) pathways were detected. In conclusion, our findings reveal antifungal-induced changes in protein abundance of C. albicans. By using the previously identified components of the BUTE of S. saponaria(e.g., saponins and sesquiterpene oligoglycosides), it will be possible to compare the behavior of compounds with unknown mechanisms of action, and this knowledge will help to focus the subsequent biochemical work aimed at defining the effects of these compounds.

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