Identification and antifungal susceptibility of Candida species isolated from the urine of patients in a university hospital in Brazil

ABSTRACT The aim of this study was to identify Candida spp. isolated from candiduria episodes at a tertiary hospital in the Midwest region of Brazil, and to determine their susceptibility profiles to antifungal compounds. From May 2011 to April 2012, Candida spp. isolated from 106 adult patients wit...

Full description

Bibliographic Details
Published in:Revista do Instituto de Medicina Tropical de São Paulo
Main Authors: Gláucia Moreira Espíndola Lima, Maína de Oliveira Nunes, Marilene Rodrigues Chang, Rosianne Assis de Sousa Tsujisaki, Joslaine de Oliveira Nunes, Cleison Ledesma Taira, Danilo Yamamoto Thomaz, Gilda Maria Bárbaro Del Negro, Rinaldo Pôncio Mendes, Anamaria Mello Miranda Paniago
Format: Article in Journal/Newspaper
Language:English
Published: Universidade de São Paulo (USP) 2017
Subjects:
Urine infections
Antifungal agents
Nosocomial candiduria
Candida species
Antifungal susceptibility
Arctic medicine. Tropical medicine
RC955-962
Infectious and parasitic diseases
RC109-216
Arctic
Online Access:https://doi.org/10.1590/s1678-9946201759075
https://doaj.org/article/08621c7e29f7462dac470f6401173a19
Description
Summary:ABSTRACT The aim of this study was to identify Candida spp. isolated from candiduria episodes at a tertiary hospital in the Midwest region of Brazil, and to determine their susceptibility profiles to antifungal compounds. From May 2011 to April 2012, Candida spp. isolated from 106 adult patients with candiduria admitted to the University Hospital of the Federal University of Mato Grosso do Sul were evaluated. Both, species identification and susceptibility testing with fluconazole-FLC, voriconazole-VRC, and amphotericin B-AmB were carried out using the Vitek 2. To discriminate species of the C. parapsilosis complex, a RAPD-PCR technique using the RPO2 primer was performed. From the total of 106 isolates, 42 (39.6%) C. albicans and 64 (60.4%) Candida non-albicans (CNA) - 33 C. tropicalis, 18 C. glabrata, 5 C. krusei, 4 C. parapsilosis sensu stricto, 2 C. kefyr, 1 C. lusitaniae, and 1 C. guilliermondii were identified. All isolates were susceptible to AmB and VRC, whereas all C. glabrata isolates presented either resistance (5.6%) or dose-dependent susceptibility (94.4%) to FLC. The study of Candida spp. and their resistance profiles may help in tailoring more efficient therapeutic strategies for candiduria.

Similar Items