Carbohydrate metabolism in Antarctic birds' erythrocytes: Levels of IP5 and 2,3-DPG and their effect on chicken hexokinase activity
The aim of the present study is to show the possible implication of organic phosphate metabolites=namely inositol pentaphosphate (IP5) and 2,3-bisphosphoglycerate (2,3-DPG)=in the glucose metabolism displayed by birds erythrocytes. For this purpose, a preparation of hexokinase (HK) has been purified...
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National Institute of Polar Research
1998
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ftdoajarticles:oai:doaj.org/article:060eda45370c443ead71b229cf28d514 2023-05-15T13:41:57+02:00 Carbohydrate metabolism in Antarctic birds' erythrocytes: Levels of IP5 and 2,3-DPG and their effect on chicken hexokinase activity Edson Rodrigues Rubens Rosa Dario Ocampos Claudete D. Rosa Metry Bacila 1998-03-01T00:00:00Z https://doi.org/10.15094/00009031 https://doaj.org/article/060eda45370c443ead71b229cf28d514 EN JA eng jpn National Institute of Polar Research http://doi.org/10.15094/00009031 https://doaj.org/toc/0085-7289 https://doaj.org/toc/2432-079X doi:10.15094/00009031 0085-7289 2432-079X https://doaj.org/article/060eda45370c443ead71b229cf28d514 Antarctic Record, Vol 42, Iss 1, Pp 101-110 (1998) Geography (General) G1-922 article 1998 ftdoajarticles https://doi.org/10.15094/00009031 2022-12-31T07:14:00Z The aim of the present study is to show the possible implication of organic phosphate metabolites=namely inositol pentaphosphate (IP5) and 2,3-bisphosphoglycerate (2,3-DPG)=in the glucose metabolism displayed by birds erythrocytes. For this purpose, a preparation of hexokinase (HK) has been purified from chicken erythrocytes (RBC) by affinity chromatography in Sepharose-N-aminohexanoyl glucosamine and used for kinetic experiments. It has been shown that IP5 is a competitive inhibitor of HK in regard to Mg^<2+>, but not in regard to glucose and MgATP^<2->. On the other hand, this preparation of HK is also competitively inhibited by ATP^<4->. It has been found that the total intracellular content of Mg^<2+> (2.76μmol/ml) is lower than the total value for the content of ATP and IP5 found in a standard packed red blood cell suspension. Article in Journal/Newspaper Antarc* Antarctic Directory of Open Access Journals: DOAJ Articles Antarctic |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English Japanese |
topic |
Geography (General) G1-922 |
spellingShingle |
Geography (General) G1-922 Edson Rodrigues Rubens Rosa Dario Ocampos Claudete D. Rosa Metry Bacila Carbohydrate metabolism in Antarctic birds' erythrocytes: Levels of IP5 and 2,3-DPG and their effect on chicken hexokinase activity |
topic_facet |
Geography (General) G1-922 |
description |
The aim of the present study is to show the possible implication of organic phosphate metabolites=namely inositol pentaphosphate (IP5) and 2,3-bisphosphoglycerate (2,3-DPG)=in the glucose metabolism displayed by birds erythrocytes. For this purpose, a preparation of hexokinase (HK) has been purified from chicken erythrocytes (RBC) by affinity chromatography in Sepharose-N-aminohexanoyl glucosamine and used for kinetic experiments. It has been shown that IP5 is a competitive inhibitor of HK in regard to Mg^<2+>, but not in regard to glucose and MgATP^<2->. On the other hand, this preparation of HK is also competitively inhibited by ATP^<4->. It has been found that the total intracellular content of Mg^<2+> (2.76μmol/ml) is lower than the total value for the content of ATP and IP5 found in a standard packed red blood cell suspension. |
format |
Article in Journal/Newspaper |
author |
Edson Rodrigues Rubens Rosa Dario Ocampos Claudete D. Rosa Metry Bacila |
author_facet |
Edson Rodrigues Rubens Rosa Dario Ocampos Claudete D. Rosa Metry Bacila |
author_sort |
Edson Rodrigues |
title |
Carbohydrate metabolism in Antarctic birds' erythrocytes: Levels of IP5 and 2,3-DPG and their effect on chicken hexokinase activity |
title_short |
Carbohydrate metabolism in Antarctic birds' erythrocytes: Levels of IP5 and 2,3-DPG and their effect on chicken hexokinase activity |
title_full |
Carbohydrate metabolism in Antarctic birds' erythrocytes: Levels of IP5 and 2,3-DPG and their effect on chicken hexokinase activity |
title_fullStr |
Carbohydrate metabolism in Antarctic birds' erythrocytes: Levels of IP5 and 2,3-DPG and their effect on chicken hexokinase activity |
title_full_unstemmed |
Carbohydrate metabolism in Antarctic birds' erythrocytes: Levels of IP5 and 2,3-DPG and their effect on chicken hexokinase activity |
title_sort |
carbohydrate metabolism in antarctic birds' erythrocytes: levels of ip5 and 2,3-dpg and their effect on chicken hexokinase activity |
publisher |
National Institute of Polar Research |
publishDate |
1998 |
url |
https://doi.org/10.15094/00009031 https://doaj.org/article/060eda45370c443ead71b229cf28d514 |
geographic |
Antarctic |
geographic_facet |
Antarctic |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_source |
Antarctic Record, Vol 42, Iss 1, Pp 101-110 (1998) |
op_relation |
http://doi.org/10.15094/00009031 https://doaj.org/toc/0085-7289 https://doaj.org/toc/2432-079X doi:10.15094/00009031 0085-7289 2432-079X https://doaj.org/article/060eda45370c443ead71b229cf28d514 |
op_doi |
https://doi.org/10.15094/00009031 |
_version_ |
1766160825959055360 |