Carbohydrate metabolism in Antarctic birds' erythrocytes: Levels of IP5 and 2,3-DPG and their effect on chicken hexokinase activity
The aim of the present study is to show the possible implication of organic phosphate metabolites=namely inositol pentaphosphate (IP5) and 2,3-bisphosphoglycerate (2,3-DPG)=in the glucose metabolism displayed by birds erythrocytes. For this purpose, a preparation of hexokinase (HK) has been purified...
| Main Authors: | , , , , |
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| Format: | Article in Journal/Newspaper |
| Language: | English Japanese |
| Published: |
National Institute of Polar Research
1998
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| Subjects: | |
| Online Access: | https://doi.org/10.15094/00009031 https://doaj.org/article/060eda45370c443ead71b229cf28d514 |
| Summary: | The aim of the present study is to show the possible implication of organic phosphate metabolites=namely inositol pentaphosphate (IP5) and 2,3-bisphosphoglycerate (2,3-DPG)=in the glucose metabolism displayed by birds erythrocytes. For this purpose, a preparation of hexokinase (HK) has been purified from chicken erythrocytes (RBC) by affinity chromatography in Sepharose-N-aminohexanoyl glucosamine and used for kinetic experiments. It has been shown that IP5 is a competitive inhibitor of HK in regard to Mg^<2+>, but not in regard to glucose and MgATP^<2->. On the other hand, this preparation of HK is also competitively inhibited by ATP^<4->. It has been found that the total intracellular content of Mg^<2+> (2.76μmol/ml) is lower than the total value for the content of ATP and IP5 found in a standard packed red blood cell suspension. |
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