Neutrophile Eisenbakterien
The aim of this work was to study a group of bacteria, which has been investigated since many years but still remains mysterious: the neutrophilic iron bacteria. These were explored on the one hand in their natural habitats, which were places in the National Park "Lower Oder Valley" in Ger...
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| Other Authors: | , , |
| Format: | Doctoral or Postdoctoral Thesis |
| Language: | German |
| Published: |
2019
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| Subjects: | |
| Online Access: | https://depositonce.tu-berlin.de/handle/11303/9459 https://doi.org/10.14279/depositonce-8515 |
| Summary: | The aim of this work was to study a group of bacteria, which has been investigated since many years but still remains mysterious: the neutrophilic iron bacteria. These were explored on the one hand in their natural habitats, which were places in the National Park "Lower Oder Valley" in Germany and newly found sites on the island Tierra del Fuego in Argentina. Furthermore there was the opportunity to take part in an expedition to search for iron bacteria on several Antarctic islands. On the other hand the isolation and characterization of iron bacteria pure cultures was a central part of this work. Various methods were applied which included classical methods of microbiology such as microscopy and cultivation as well as methods of modern molecular biology. These included: Microscopic analysis of environmental samples Generation of clone libraries to study microbial communities Development of adapted isolation media and suitable conditions Morphological characterization of pure cultures with light, epifluorescence, scanning electron and confocal laser scanning microscopy Sequencing of the 16S rDNA and other genes to clarify the phylogenetic relationship of the isolates Growth experiments under defined cultivation conditions Gradient culture of microaerophilic iron bacteria Using these different methods comprehensive results were obtained. In addition to already known iron bacteria habitats in Germany, many different, sometimes extensive sites were found and investigated in Tierra del Fuego. The found iron bacteria were assigned microscopically to morphotypes, which were already described from the northern hemisphere. A large number and diversity of iron and manganese depositing bacteria has been isolated. Many of them represent new genera, species or subspecies, since they differed to a varying extent from already described strains, which was evident especially from sequence comparisons. Furthermore culture conditions have been developed which made isolation and cultivation under "near-natural" conditions ... |
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