Spermatogenesis in the blue swimming crab, Portunus pelagicus, and evidence for histones in mature sperm nuclei

Spermatogenesis in the blue swimming crab, Portunus pelagicus , is described by light and electron microscopy. The testis is composed of anterior (AT) and posterior (PT) lobes, that are partitioned into lobules by connective tissue trabecula, and further divided into zones (germinal, transformation...

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Bibliographic Details
Main Authors: Michael Stewart, P Stewart, N Soonklang, V Linthong, Peter Hanna, Wei Duan, P Sobhon
Format: Other Non-Article Part of Journal/Newspaper
Language:unknown
Published: 2010
Subjects:
Uncategorized
Portunus pelagicus
spermatogenesis
spermatozoa
ultrastructure
basic nuclear proteins
Science & Technology
Life Sciences & Biomedicine
Anatomy & Morphology
Cell Biology
MALE REPRODUCTIVE-SYSTEM
FRESH-WATER PRAWN
CHIONOECETES-OPILIO
ELECTRON-MICROSCOPY
VAS-DEFERENS
SPIDER CRAB
LONGIROSTRIS CRUSTACEA
FINE-STRUCTURE
FIDDLER-CRAB
Chionoecetes opilio
Online Access:http://hdl.handle.net/10536/DRO/DU:30031456
https://figshare.com/articles/journal_contribution/Spermatogenesis_in_the_blue_swimming_crab_Portunus_pelagicus_and_evidence_for_histones_in_mature_sperm_nuclei/21020191
Description
Summary:Spermatogenesis in the blue swimming crab, Portunus pelagicus , is described by light and electron microscopy. The testis is composed of anterior (AT) and posterior (PT) lobes, that are partitioned into lobules by connective tissue trabecula, and further divided into zones (germinal, transformation and evacuation), each with various stages of cellular differentiation. The vas deferens is classified into three distinct regions: anterior (AVD), median (MVD), and posterior (PVD), on the presence of spermatophores and two secretions, termed substance I and II. Based on the degree and patterns of heterochromatin, spermatogenesis is classified into 13 stages: two spermatogonia (SgA and SgB), six primary spermatocytes (leptotene, zygotene, pachytene, diplotene, diakinesis, and metaphase), a secondary spermatocyte (SSc), three spermatids (St 1–3), and a mature spermatozoon. Spermatid stages are differentiated by chromatin decondensation and the formation of an acrosomal complex, which is unique to brachyurans. Mature spermatozoa are aflagellated, and have a nuclear projection and a spherical acrosome. AUT-PAGE and Western blots show that, during chromatin decondensation, there is a reduction of most histones, with only small amounts of H2B and H3 remaining in mature spermatozoa.

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