Trapping and imaging of micron-sized embryos using dielectrophoresis

• Main Authors: Khashayar Khoshmanesh, N Kiss, Saeid Nahavandi, C Evans, J Cooper, D Williams, D Wlodkowic
• Format: Other Non-Article Part of Journal/Newspaper
• Language: unknown
• Published: 2011
• Subjects: Uncategorized; dielecrophoresis; embryo; lab-on-a-chip; microfluidics; real-time imaging; Science & Technology; Life Sciences & Biomedicine; Physical Sciences; Biochemical Research Methods; Chemistry; Analytical; Biochemistry & Molecular Biology; CAENORHABDITIS-ELEGANS; C. ELEGANS; MODEL; MANIPULATION; DISCOVERY; CHIP; CELL; Antarctic; Antarc*
• Online Access: http://hdl.handle.net/10536/DRO/DU:30044163; https://figshare.com/articles/journal_contribution/Trapping_and_imaging_of_micron-sized_embryos_using_dielectrophoresis/20993899

Development of dielectrophoretic (DEP) arrays for real-time imaging of embryonic organisms is described. Microelectrode arrays were used for trapping both embryonated eggs and larval stages of Antarctic nematode Panagrolaimus davidi. Ellipsoid single-shell model was also applied to study the interactions between DEP fields and developing multicellular organisms. This work provides proof-of-concept application of chip-based technologies for the analysis of individual embryos trapped under DEP force.