Trapping and imaging of micron-sized embryos using dielectrophoresis

Development of dielectrophoretic (DEP) arrays for real-time imaging of embryonic organisms is described. Microelectrode arrays were used for trapping both embryonated eggs and larval stages of Antarctic nematode Panagrolaimus davidi. Ellipsoid single-shell model was also applied to study the interac...

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Bibliographic Details
Main Authors: Khashayar Khoshmanesh, N Kiss, Saeid Nahavandi, C Evans, J Cooper, D Williams, D Wlodkowic
Format: Other Non-Article Part of Journal/Newspaper
Language:unknown
Published: 2011
Subjects:
Uncategorized
dielecrophoresis
embryo
lab-on-a-chip
microfluidics
real-time imaging
Science & Technology
Life Sciences & Biomedicine
Physical Sciences
Biochemical Research Methods
Chemistry
Analytical
Biochemistry & Molecular Biology
CAENORHABDITIS-ELEGANS
C. ELEGANS
MODEL
MANIPULATION
DISCOVERY
CHIP
CELL
Antarctic
Antarc*
Online Access:http://hdl.handle.net/10536/DRO/DU:30044163
https://figshare.com/articles/journal_contribution/Trapping_and_imaging_of_micron-sized_embryos_using_dielectrophoresis/20993899
Description
Summary:Development of dielectrophoretic (DEP) arrays for real-time imaging of embryonic organisms is described. Microelectrode arrays were used for trapping both embryonated eggs and larval stages of Antarctic nematode Panagrolaimus davidi. Ellipsoid single-shell model was also applied to study the interactions between DEP fields and developing multicellular organisms. This work provides proof-of-concept application of chip-based technologies for the analysis of individual embryos trapped under DEP force.

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