The effect of Ca2+, Cd2+ and Ni2+ on detergent-permeabilized vascular smooth muscle from the shark, Squalus acanthias
We examined the effect of Ca2+, Cd2+, or Ni2+ on vascular smooth muscle intracellular proteins involved in contraction, using rings of detergent-permeabilized aortae from the spiny dogfish, Squalus acanthias. Addition of Ca2+ stimulated contraction of the vascular smooth muscle, and permeabilization...
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ftdeakinunifig:oai:figshare.com:article/20868067 2024-06-23T07:57:32+00:00 The effect of Ca2+, Cd2+ and Ni2+ on detergent-permeabilized vascular smooth muscle from the shark, Squalus acanthias DH Evans E Chipouras T Toop John Donald 1993-10-25T00:00:00Z http://hdl.handle.net/10536/DRO/DU:30094419 https://figshare.com/articles/journal_contribution/The_effect_of_Ca2_Cd2_and_Ni2_on_detergent-permeabilized_vascular_smooth_muscle_from_the_shark_Squalus_acanthias/20868067 unknown http://hdl.handle.net/10536/DRO/DU:30094419 https://figshare.com/articles/journal_contribution/The_effect_of_Ca2_Cd2_and_Ni2_on_detergent-permeabilized_vascular_smooth_muscle_from_the_shark_Squalus_acanthias/20868067 All Rights Reserved Pharmacology and pharmaceutical sciences not elsewhere classified Animals Aorta Cadmium Calcium In Vitro Techniques Nickel Octoxynol Sharks Vascular smooth muscle Detergent-permeabilized Text Journal contribution 1993 ftdeakinunifig 2024-06-06T02:00:31Z We examined the effect of Ca2+, Cd2+, or Ni2+ on vascular smooth muscle intracellular proteins involved in contraction, using rings of detergent-permeabilized aortae from the spiny dogfish, Squalus acanthias. Addition of Ca2+ stimulated contraction of the vascular smooth muscle, and permeabilization by treatment with Triton X-100 increased the sensitivity to Ca2+ nearly 5 log units, demonstrating that this protocol left contractile and regulatory proteins intact. Addition of 1 microM calmodulin did not increase the sensitivity of the rings to Ca2+, suggesting that this preparation is not leaky to this regulatory protein. Neither Cd2+ nor Ni2+ stimulated contraction of permeabilized rings demonstrating that the previously-described contractile action of these heavy metals is not mediated by direct stimulation of intracellular proteins, rather by interaction with sarcolemmal proteins. Article in Journal/Newspaper spiny dogfish Squalus acanthias DRO - Deakin Research Online Triton ENVELOPE(-55.615,-55.615,49.517,49.517) |
institution |
Open Polar |
collection |
DRO - Deakin Research Online |
op_collection_id |
ftdeakinunifig |
language |
unknown |
topic |
Pharmacology and pharmaceutical sciences not elsewhere classified Animals Aorta Cadmium Calcium In Vitro Techniques Nickel Octoxynol Sharks Vascular smooth muscle Detergent-permeabilized |
spellingShingle |
Pharmacology and pharmaceutical sciences not elsewhere classified Animals Aorta Cadmium Calcium In Vitro Techniques Nickel Octoxynol Sharks Vascular smooth muscle Detergent-permeabilized DH Evans E Chipouras T Toop John Donald The effect of Ca2+, Cd2+ and Ni2+ on detergent-permeabilized vascular smooth muscle from the shark, Squalus acanthias |
topic_facet |
Pharmacology and pharmaceutical sciences not elsewhere classified Animals Aorta Cadmium Calcium In Vitro Techniques Nickel Octoxynol Sharks Vascular smooth muscle Detergent-permeabilized |
description |
We examined the effect of Ca2+, Cd2+, or Ni2+ on vascular smooth muscle intracellular proteins involved in contraction, using rings of detergent-permeabilized aortae from the spiny dogfish, Squalus acanthias. Addition of Ca2+ stimulated contraction of the vascular smooth muscle, and permeabilization by treatment with Triton X-100 increased the sensitivity to Ca2+ nearly 5 log units, demonstrating that this protocol left contractile and regulatory proteins intact. Addition of 1 microM calmodulin did not increase the sensitivity of the rings to Ca2+, suggesting that this preparation is not leaky to this regulatory protein. Neither Cd2+ nor Ni2+ stimulated contraction of permeabilized rings demonstrating that the previously-described contractile action of these heavy metals is not mediated by direct stimulation of intracellular proteins, rather by interaction with sarcolemmal proteins. |
format |
Article in Journal/Newspaper |
author |
DH Evans E Chipouras T Toop John Donald |
author_facet |
DH Evans E Chipouras T Toop John Donald |
author_sort |
DH Evans |
title |
The effect of Ca2+, Cd2+ and Ni2+ on detergent-permeabilized vascular smooth muscle from the shark, Squalus acanthias |
title_short |
The effect of Ca2+, Cd2+ and Ni2+ on detergent-permeabilized vascular smooth muscle from the shark, Squalus acanthias |
title_full |
The effect of Ca2+, Cd2+ and Ni2+ on detergent-permeabilized vascular smooth muscle from the shark, Squalus acanthias |
title_fullStr |
The effect of Ca2+, Cd2+ and Ni2+ on detergent-permeabilized vascular smooth muscle from the shark, Squalus acanthias |
title_full_unstemmed |
The effect of Ca2+, Cd2+ and Ni2+ on detergent-permeabilized vascular smooth muscle from the shark, Squalus acanthias |
title_sort |
effect of ca2+, cd2+ and ni2+ on detergent-permeabilized vascular smooth muscle from the shark, squalus acanthias |
publishDate |
1993 |
url |
http://hdl.handle.net/10536/DRO/DU:30094419 https://figshare.com/articles/journal_contribution/The_effect_of_Ca2_Cd2_and_Ni2_on_detergent-permeabilized_vascular_smooth_muscle_from_the_shark_Squalus_acanthias/20868067 |
long_lat |
ENVELOPE(-55.615,-55.615,49.517,49.517) |
geographic |
Triton |
geographic_facet |
Triton |
genre |
spiny dogfish Squalus acanthias |
genre_facet |
spiny dogfish Squalus acanthias |
op_relation |
http://hdl.handle.net/10536/DRO/DU:30094419 https://figshare.com/articles/journal_contribution/The_effect_of_Ca2_Cd2_and_Ni2_on_detergent-permeabilized_vascular_smooth_muscle_from_the_shark_Squalus_acanthias/20868067 |
op_rights |
All Rights Reserved |
_version_ |
1802651222308552704 |