The effect of Ca2+, Cd2+ and Ni2+ on detergent-permeabilized vascular smooth muscle from the shark, Squalus acanthias

We examined the effect of Ca2+, Cd2+, or Ni2+ on vascular smooth muscle intracellular proteins involved in contraction, using rings of detergent-permeabilized aortae from the spiny dogfish, Squalus acanthias. Addition of Ca2+ stimulated contraction of the vascular smooth muscle, and permeabilization...

Full description

Bibliographic Details
Main Authors: DH Evans, E Chipouras, T Toop, John Donald
Format: Article in Journal/Newspaper
Language:unknown
Published: 1993
Subjects:
Pharmacology and pharmaceutical sciences not elsewhere classified
Animals
Aorta
Cadmium
Calcium
In Vitro Techniques
Nickel
Octoxynol
Sharks
Vascular smooth muscle
Detergent-permeabilized
Triton
spiny dogfish
Squalus acanthias
Online Access:http://hdl.handle.net/10536/DRO/DU:30094419
https://figshare.com/articles/journal_contribution/The_effect_of_Ca2_Cd2_and_Ni2_on_detergent-permeabilized_vascular_smooth_muscle_from_the_shark_Squalus_acanthias/20868067
Description
Summary:We examined the effect of Ca2+, Cd2+, or Ni2+ on vascular smooth muscle intracellular proteins involved in contraction, using rings of detergent-permeabilized aortae from the spiny dogfish, Squalus acanthias. Addition of Ca2+ stimulated contraction of the vascular smooth muscle, and permeabilization by treatment with Triton X-100 increased the sensitivity to Ca2+ nearly 5 log units, demonstrating that this protocol left contractile and regulatory proteins intact. Addition of 1 microM calmodulin did not increase the sensitivity of the rings to Ca2+, suggesting that this preparation is not leaky to this regulatory protein. Neither Cd2+ nor Ni2+ stimulated contraction of permeabilized rings demonstrating that the previously-described contractile action of these heavy metals is not mediated by direct stimulation of intracellular proteins, rather by interaction with sarcolemmal proteins.

Similar Items