Publisher Correction: Discordance for genotypic sex in phenotypic female Atlantic salmon (Salmo salar) is related to a reduced sdY copy number (Scientific Reports, (2020), 10, 1, (9651), 10.1038/s41598-020-66406-x)
AbstractThe master sex determinant in rainbow trout (Oncorhynchus mykiss), sexually dimorphic on the Y chromosome (sdY), is strongly but not perfectly associated with male phenotype in several other species from the family Salmonidae. Currently, the cause and implications of discordance for sdY-pred...
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ftdeakinunifig:oai:figshare.com:article/20702023 2024-09-09T19:30:25+00:00 Publisher Correction: Discordance for genotypic sex in phenotypic female Atlantic salmon (Salmo salar) is related to a reduced sdY copy number (Scientific Reports, (2020), 10, 1, (9651), 10.1038/s41598-020-66406-x) MS Brown BS Evans Luis Afonso 2020-12-01T00:00:00Z http://hdl.handle.net/10536/DRO/DU:30139141 https://figshare.com/articles/journal_contribution/Discordance_for_genotypic_sex_in_phenotypic_female_Atlantic_salmon_Salmo_salar_is_related_to_a_reduced_sdY_copy_number/20702023 unknown http://hdl.handle.net/10536/DRO/DU:30139141 https://figshare.com/articles/journal_contribution/Discordance_for_genotypic_sex_in_phenotypic_female_Atlantic_salmon_Salmo_salar_is_related_to_a_reduced_sdY_copy_number/20702023 CC BY 4.0 Fisheries sciences not elsewhere classified Genetics not elsewhere classified Animal physiology - systems Zoology not elsewhere classified Science & Technology Multidisciplinary Sciences Science & Technology - Other Topics CHINOOK SALMON ONCORHYNCHUS-TSHAWYTSCHA DETERMINING GENE RAINBOW-TROUT COHO SALMON DNA MARKERS IDENTIFICATION PSEUDOGENE LOCI FISH School of Life and Environmental Sciences Centre for Integrative Ecology MD Multidisciplinary 3005 Fisheries sciences 3105 Genetics Text Journal contribution 2020 ftdeakinunifig 2024-06-20T00:38:39Z AbstractThe master sex determinant in rainbow trout (Oncorhynchus mykiss), sexually dimorphic on the Y chromosome (sdY), is strongly but not perfectly associated with male phenotype in several other species from the family Salmonidae. Currently, the cause and implications of discordance for sdY-predicted genotypic sex and phenotypic sex in these species is unclear. Using an established multiplex PCR test for exons 2 and 3 of sdY, we demonstrated that sdY-predicted genotypic sex was discordant with histologically evidenced phenotypic sex in 4% of 176 Tasmanian Atlantic salmon. All discordant individuals were phenotypic females presenting a male genotype. Using real-time qPCR assays that we developed and validated for exons 2, 3 and 4 of sdY, all genotype-phenotype discordant females were confirmed to possess sdY, albeit at a reduced number of copies when compared to phenotypic males. The real-time qPCR assays also demonstrated reduced levels of sdY in 30% of phenotypic females that the established multiplex PCR-based test indicated to be devoid of sdY. These findings suggest sdY may be reduced in copy number or mosaicked in the genomic DNA of sdY-positive phenotypic female Atlantic salmon and highlight the importance of understanding the effects of reduced sdY copies on the development of phenotypic sex. Article in Journal/Newspaper Atlantic salmon Salmo salar DRO - Deakin Research Online The ''Y'' ENVELOPE(-112.453,-112.453,57.591,57.591) |
institution |
Open Polar |
collection |
DRO - Deakin Research Online |
op_collection_id |
ftdeakinunifig |
language |
unknown |
topic |
Fisheries sciences not elsewhere classified Genetics not elsewhere classified Animal physiology - systems Zoology not elsewhere classified Science & Technology Multidisciplinary Sciences Science & Technology - Other Topics CHINOOK SALMON ONCORHYNCHUS-TSHAWYTSCHA DETERMINING GENE RAINBOW-TROUT COHO SALMON DNA MARKERS IDENTIFICATION PSEUDOGENE LOCI FISH School of Life and Environmental Sciences Centre for Integrative Ecology MD Multidisciplinary 3005 Fisheries sciences 3105 Genetics |
spellingShingle |
Fisheries sciences not elsewhere classified Genetics not elsewhere classified Animal physiology - systems Zoology not elsewhere classified Science & Technology Multidisciplinary Sciences Science & Technology - Other Topics CHINOOK SALMON ONCORHYNCHUS-TSHAWYTSCHA DETERMINING GENE RAINBOW-TROUT COHO SALMON DNA MARKERS IDENTIFICATION PSEUDOGENE LOCI FISH School of Life and Environmental Sciences Centre for Integrative Ecology MD Multidisciplinary 3005 Fisheries sciences 3105 Genetics MS Brown BS Evans Luis Afonso Publisher Correction: Discordance for genotypic sex in phenotypic female Atlantic salmon (Salmo salar) is related to a reduced sdY copy number (Scientific Reports, (2020), 10, 1, (9651), 10.1038/s41598-020-66406-x) |
topic_facet |
Fisheries sciences not elsewhere classified Genetics not elsewhere classified Animal physiology - systems Zoology not elsewhere classified Science & Technology Multidisciplinary Sciences Science & Technology - Other Topics CHINOOK SALMON ONCORHYNCHUS-TSHAWYTSCHA DETERMINING GENE RAINBOW-TROUT COHO SALMON DNA MARKERS IDENTIFICATION PSEUDOGENE LOCI FISH School of Life and Environmental Sciences Centre for Integrative Ecology MD Multidisciplinary 3005 Fisheries sciences 3105 Genetics |
description |
AbstractThe master sex determinant in rainbow trout (Oncorhynchus mykiss), sexually dimorphic on the Y chromosome (sdY), is strongly but not perfectly associated with male phenotype in several other species from the family Salmonidae. Currently, the cause and implications of discordance for sdY-predicted genotypic sex and phenotypic sex in these species is unclear. Using an established multiplex PCR test for exons 2 and 3 of sdY, we demonstrated that sdY-predicted genotypic sex was discordant with histologically evidenced phenotypic sex in 4% of 176 Tasmanian Atlantic salmon. All discordant individuals were phenotypic females presenting a male genotype. Using real-time qPCR assays that we developed and validated for exons 2, 3 and 4 of sdY, all genotype-phenotype discordant females were confirmed to possess sdY, albeit at a reduced number of copies when compared to phenotypic males. The real-time qPCR assays also demonstrated reduced levels of sdY in 30% of phenotypic females that the established multiplex PCR-based test indicated to be devoid of sdY. These findings suggest sdY may be reduced in copy number or mosaicked in the genomic DNA of sdY-positive phenotypic female Atlantic salmon and highlight the importance of understanding the effects of reduced sdY copies on the development of phenotypic sex. |
format |
Article in Journal/Newspaper |
author |
MS Brown BS Evans Luis Afonso |
author_facet |
MS Brown BS Evans Luis Afonso |
author_sort |
MS Brown |
title |
Publisher Correction: Discordance for genotypic sex in phenotypic female Atlantic salmon (Salmo salar) is related to a reduced sdY copy number (Scientific Reports, (2020), 10, 1, (9651), 10.1038/s41598-020-66406-x) |
title_short |
Publisher Correction: Discordance for genotypic sex in phenotypic female Atlantic salmon (Salmo salar) is related to a reduced sdY copy number (Scientific Reports, (2020), 10, 1, (9651), 10.1038/s41598-020-66406-x) |
title_full |
Publisher Correction: Discordance for genotypic sex in phenotypic female Atlantic salmon (Salmo salar) is related to a reduced sdY copy number (Scientific Reports, (2020), 10, 1, (9651), 10.1038/s41598-020-66406-x) |
title_fullStr |
Publisher Correction: Discordance for genotypic sex in phenotypic female Atlantic salmon (Salmo salar) is related to a reduced sdY copy number (Scientific Reports, (2020), 10, 1, (9651), 10.1038/s41598-020-66406-x) |
title_full_unstemmed |
Publisher Correction: Discordance for genotypic sex in phenotypic female Atlantic salmon (Salmo salar) is related to a reduced sdY copy number (Scientific Reports, (2020), 10, 1, (9651), 10.1038/s41598-020-66406-x) |
title_sort |
publisher correction: discordance for genotypic sex in phenotypic female atlantic salmon (salmo salar) is related to a reduced sdy copy number (scientific reports, (2020), 10, 1, (9651), 10.1038/s41598-020-66406-x) |
publishDate |
2020 |
url |
http://hdl.handle.net/10536/DRO/DU:30139141 https://figshare.com/articles/journal_contribution/Discordance_for_genotypic_sex_in_phenotypic_female_Atlantic_salmon_Salmo_salar_is_related_to_a_reduced_sdY_copy_number/20702023 |
long_lat |
ENVELOPE(-112.453,-112.453,57.591,57.591) |
geographic |
The ''Y'' |
geographic_facet |
The ''Y'' |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_relation |
http://hdl.handle.net/10536/DRO/DU:30139141 https://figshare.com/articles/journal_contribution/Discordance_for_genotypic_sex_in_phenotypic_female_Atlantic_salmon_Salmo_salar_is_related_to_a_reduced_sdY_copy_number/20702023 |
op_rights |
CC BY 4.0 |
_version_ |
1809899400358526976 |