Publisher Correction: Discordance for genotypic sex in phenotypic female Atlantic salmon (Salmo salar) is related to a reduced sdY copy number (Scientific Reports, (2020), 10, 1, (9651), 10.1038/s41598-020-66406-x)

• Main Authors: MS Brown, BS Evans, Luis Afonso
• Format: Article in Journal/Newspaper
• Language: unknown
• Published: 2020
• Subjects: Fisheries sciences not elsewhere classified; Genetics not elsewhere classified; Animal physiology - systems; Zoology not elsewhere classified; Science & Technology; Multidisciplinary Sciences; Science & Technology - Other Topics; CHINOOK SALMON; ONCORHYNCHUS-TSHAWYTSCHA; DETERMINING GENE; RAINBOW-TROUT; COHO SALMON; DNA MARKERS; IDENTIFICATION; PSEUDOGENE; LOCI; FISH; School of Life and Environmental Sciences; Centre for Integrative Ecology; MD Multidisciplinary; 3005 Fisheries sciences; 3105 Genetics; The ''Y''; Atlantic salmon; Salmo salar
• Online Access: http://hdl.handle.net/10536/DRO/DU:30139141; https://figshare.com/articles/journal_contribution/Discordance_for_genotypic_sex_in_phenotypic_female_Atlantic_salmon_Salmo_salar_is_related_to_a_reduced_sdY_copy_number/20702023

AbstractThe master sex determinant in rainbow trout (Oncorhynchus mykiss), sexually dimorphic on the Y chromosome (sdY), is strongly but not perfectly associated with male phenotype in several other species from the family Salmonidae. Currently, the cause and implications of discordance for sdY-predicted genotypic sex and phenotypic sex in these species is unclear. Using an established multiplex PCR test for exons 2 and 3 of sdY, we demonstrated that sdY-predicted genotypic sex was discordant with histologically evidenced phenotypic sex in 4% of 176 Tasmanian Atlantic salmon. All discordant individuals were phenotypic females presenting a male genotype. Using real-time qPCR assays that we developed and validated for exons 2, 3 and 4 of sdY, all genotype-phenotype discordant females were confirmed to possess sdY, albeit at a reduced number of copies when compared to phenotypic males. The real-time qPCR assays also demonstrated reduced levels of sdY in 30% of phenotypic females that the established multiplex PCR-based test indicated to be devoid of sdY. These findings suggest sdY may be reduced in copy number or mosaicked in the genomic DNA of sdY-positive phenotypic female Atlantic salmon and highlight the importance of understanding the effects of reduced sdY copies on the development of phenotypic sex.