Macrococcus canis and M. caseolyticus in dogs: occurrence, genetic diversity and antibiotic resistance. ...
BACKGROUND The discovery of a new Macrococcus canis species isolated from skin and infection sites of dogs led us to question if Macrococcus spp. are common in dogs and are resistant to antibiotics. HYPOTHESIS/OBJECTIVES To evaluate the occurrence of Macrococcus spp. in dogs, determine antibiotic re...
| Main Authors: | , , , , , , , |
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| Format: | Text |
| Language: | English |
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Blackwell Science
2017
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| Subjects: | |
| Online Access: | https://dx.doi.org/10.7892/boris.112830 https://boris.unibe.ch/112830/ |
| Summary: | BACKGROUND The discovery of a new Macrococcus canis species isolated from skin and infection sites of dogs led us to question if Macrococcus spp. are common in dogs and are resistant to antibiotics. HYPOTHESIS/OBJECTIVES To evaluate the occurrence of Macrococcus spp. in dogs, determine antibiotic resistance profiles and genetic relationships. ANIMALS One hundred and sixty two dogs (mainly West Highland white terriers and Newfoundland dogs) were screened for the presence of Macrococcus, including six dogs with Macrococcus infections. METHODS Samples were taken from skin, ear canal and oral mucosa using swabs. Macrococci were identified by matrix-assisted laser desorption ionization-time of flight mass spectrometry, 16S rRNA sequencing and nuc-PCR. Minimal inhibitory concentrations of 19 antibiotics were determined using broth microdilution. Resistance mechanisms were identified by microarray and sequencing of the fluoroquinolone-determining region of gyrA and grlA. Sequence type (ST) was determined by ... |
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