Evaluating resistance to infectious pancreatic necrosis virus in rainbow trout (Oncorhynchus mykiss) cells using CRISPR/Cas editing ...
Infectious Pancreatic Necrosis (IPN) is a highly transmissible viral disease which constitutes a major constraint in the production of cultured salmonid species worldwide, causing varying mortality rates and important economic losses in several countries. Previous studies focusing on the genetic bas...
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| Format: | Article in Journal/Newspaper |
| Language: | English |
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The University of Edinburgh
2021
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| Online Access: | https://dx.doi.org/10.7488/era/5642 https://era.ed.ac.uk/handle/1842/43099 |
| Summary: | Infectious Pancreatic Necrosis (IPN) is a highly transmissible viral disease which constitutes a major constraint in the production of cultured salmonid species worldwide, causing varying mortality rates and important economic losses in several countries. Previous studies focusing on the genetic basis of IPNV (Infectious Pancreatic Necrosis Virus) resistance in Atlantic salmon discovered a QTL of major effect, which has been exploited to improve resistance of Atlantic salmon stocks using marker-assisted selection. While this QTL effect has not been observed in other salmonids, genome editing may have potential for transferring IPNV-resistance alleles or mechanisms to other species, such as rainbow trout, the salmonid with the second largest production.Systems involving the clustered regularly interspaced short palindromic repeats (CRISPR) and its associated proteins (Cas) have become the most reliable tools for gene editing and assessment of gene function, both in cell lines and in vivo. In vitro models ... |
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