Supplementary material from "Mitochondrial replication's role in vertebrate mtDNA strand asymmetry" ...

• Main Authors: Gomes-dos-Santos, André, Vilas-Arrondo, Nair, Machado, André M., Román-Marcote, Esther, Del Río Iglesias, Jose Luís, Baldó, Francisco, Pérez, Montse, Fonseca, Miguel M., Castro, L. Filipe C., Froufe, Elsa
• Format: Article in Journal/Newspaper
• Language: unknown
• Published: The Royal Society 2023
• Subjects: Genetics; FOS Biological sciences; Genomics; Plant cell and molecular biology; Animal cell and molecular biology; Antarctic; Antarc*
• Online Access: https://dx.doi.org/10.6084/m9.figshare.c.6960931.v2; https://rs.figshare.com/collections/Supplementary_material_from_Mitochondrial_replication_s_role_in_vertebrate_mtDNA_strand_asymmetry_/6960931/2

Mitogenomes are defined as compact and structurally stable over aeons. This perception results from a vertebrate-centric vision, where few types of mtDNA rearrangements are described. Here, we bring a new light to the involvement of mitochondrial replication in the strand asymmetry of the vertebrate mtDNA. Using several species of deep-sea hatchetfish (Sternoptychidae) displaying distinct mtDNA structural arrangements, we unravel the inversion of the coding direction of protein-coding genes (PCGs). This unexpected change is coupled with a strand asymmetry nucleotide composition reversal and is shown to be directly related to the strand location of the Control Region (CR). An analysis of the fourfold redundant sites of the PCGs (greater than 6000 vertebrates), revealed the rarity of this phenomenon, found in nine fish species (five deep-sea hatchetfish). Curiously, in Antarctic notothenioid fishes (Trematominae), where a single PCG inversion (the only other record in fish) is coupled with the inversion of the ...