Supplementary material from "Pre-fertilization gamete thermal environment influences reproductive success, unmasking opposing sex-specific responses in Atlantic salmon ( Salmo salar )" ...

• Main Authors: Graziano, Marco, Solberg, Monica F., Glover, Kevin A., Vasudeva, Ramakrishnan, Dyrhovden, Lise, Murray, David, Immler, Simone, Gage, Matthew J. G.
• Format: Article in Journal/Newspaper
• Language: unknown
• Published: The Royal Society 2023
• Subjects: Ecology not elsewhere classified; Evolution of developmental systems; Evolutionary biology not elsewhere classified; Atlantic salmon; Salmo salar
• Online Access: https://dx.doi.org/10.6084/m9.figshare.c.6958228; https://rs.figshare.com/collections/Supplementary_material_from_Pre-fertilization_gamete_thermal_environment_influences_reproductive_success_unmasking_opposing_sex-specific_responses_in_Atlantic_Salmon_i_Salmo_salar_i_/6958228

The environment gametes perform in just before fertilization is increasingly recognized to affect offspring fitness, yet the contributions of male and female gametes and their adaptive significance remain largely unexplored. Here, we investigated gametic thermal plasticity and its effects on hatching success and embryo performance in Atlantic salmon ( Salmo salar ). Eggs and sperm were incubated overnight at 2°C or 8°C, temperatures within the optimal thermal range of this species. Crosses between warm- and cold-incubated gametes were compared using a full-factorial design, with half of each clutch reared in cold temperatures and the other in warm temperatures. This allowed disentangling single-sex interaction effects when pre- fertilization temperature of gametes mismatched embryonic conditions. Pre–fertilization temperature influenced hatch timing and synchrony, and matching sperm and embryo temperatures resulted in earlier hatching. Warm incubation benefited eggs but harmed sperm, reducing the hatching ...