Seasonal patterns in microbial carbon and iron transporter expression in the Southern Ocean ...
Abstract Background Heterotrophic microbes in the Southern Ocean are challenged by the double constraint of low concentrations of organic carbon (C) and iron (Fe). These essential elements are tightly coupled in cellular processes; however, the prokaryotic requirements of C and Fe under varying envi...
| Main Authors: | , , , , |
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| Format: | Article in Journal/Newspaper |
| Language: | unknown |
| Published: |
figshare
2023
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| Subjects: | |
| Online Access: | https://dx.doi.org/10.6084/m9.figshare.c.6796896.v1 https://springernature.figshare.com/collections/Seasonal_patterns_in_microbial_carbon_and_iron_transporter_expression_in_the_Southern_Ocean/6796896/1 |
| Summary: | Abstract Background Heterotrophic microbes in the Southern Ocean are challenged by the double constraint of low concentrations of organic carbon (C) and iron (Fe). These essential elements are tightly coupled in cellular processes; however, the prokaryotic requirements of C and Fe under varying environmental settings remain poorly studied. Here, we used a combination of metatranscriptomics and metaproteomics to identify prokaryotic membrane transporters for organic substrates and Fe in naturally iron-fertilized and high-nutrient, low-chlorophyll waters of the Southern Ocean during spring and late summer. Results Pronounced differences in membrane transporter profiles between seasons were observed at both sites, both at the transcript and protein level. When specific compound classes were considered, the two approaches revealed different patterns. At the transcript level, seasonal patterns were only observed for subsets of genes belonging to each transporter category. At the protein level, membrane ... |
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