A simple method for taxon-specific purification of diatom frustules from ocean sediments using a cell sorter ...
Abstract It is necessary to purify diatom frustules by taxon to perform accurate geochemical analyses of diatom fossils preserved in sediments. However, the small size of diatoms has hitherto prevented taxon-specific purification; therefore, previous geochemical analyses of diatom frustules have bee...
| Main Authors: | , , , , |
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| Format: | Article in Journal/Newspaper |
| Language: | unknown |
| Published: |
figshare
2024
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| Subjects: | |
| Online Access: | https://dx.doi.org/10.6084/m9.figshare.c.6591229.v1 https://springernature.figshare.com/collections/A_simple_method_for_taxon-specific_purification_of_diatom_frustules_from_ocean_sediments_using_a_cell_sorter/6591229/1 |
| Summary: | Abstract It is necessary to purify diatom frustules by taxon to perform accurate geochemical analyses of diatom fossils preserved in sediments. However, the small size of diatoms has hitherto prevented taxon-specific purification; therefore, previous geochemical analyses of diatom frustules have been performed with mixtures of various taxa. In this study, we developed a taxon-selective collection method of diatom fossils that uses a cell sorter. The experimental material comprised six samples from a sediment core of Hole U1538A in the Scotia Sea, Atlantic sector of the Southern Ocean drilled during the International Ocean Discovery Program Expedition 382. Following conventional pretreatments (removal of organic matter and carbonates as well as clay minerals), we conducted cell sorter experiments focusing on the optical and fluorescence characteristics of the diatom fossils. We succeeded in selectively isolating with high purity five diatom taxa representing the Southern Ocean diatom flora: (1) discoid ... |
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