Supplementary material from "Assimilation and turnover rates of lipid compounds in dominant Antarctic copepods fed with 13 C-enriched diatoms"

• Main Authors: Graeve, Martin, Boissonnot, Lauris, Niehoff, Barbara, Hagen, Wilhelm, Kattner, Gerhard
• Format: Article in Journal/Newspaper
• Language: unknown
• Published: The Royal Society 2020
• Subjects: Biochemistry; Physiology; FOS Biological sciences; Environmental Science; Antarctic; Antarc*; Copepods
• Online Access: https://dx.doi.org/10.6084/m9.figshare.c.4978292; https://rs.figshare.com/collections/Supplementary_material_from_Assimilation_and_turnover_rates_of_lipid_compounds_in_dominant_Antarctic_copepods_fed_with_sup_13_sup_C-enriched_diatoms_/4978292

The study revealed species- and stage-specific differences in lipid accumulation of the dominant Antarctic copepods, the primarily herbivorous Calanoides acutus (copepodite stage V (CV), females) and the more omnivorous Calanus propinquus (females) storing wax esters and triacylglycerols, respectively, which were collected in summer (end of December). Feeding carbon-labelled diatoms to these copepods, 13 C elucidated assimilation and turnover rates of copepod total lipids as well as specific fatty acids and alcohols. The 13 C incorporation was monitored by compound-specific stable isotope analysis (CSIA). CV stages of C. acutus exhibited an intense total lipid turnover and 55% of total lipids were labelled after 9 days of feeding. By contrast, total lipid assimilation of female C. acutus and C. propinquus was lower with 29% and 32%, respectively. The major dietary fatty acids 16 : 0, 16 : 1(n − 7) and 20 : 5(n − 3) had high turnover rates in all specimens. In C. acutus CV, the high rates of the de novo synthesized long-chain monounsaturated fatty acids and alcohols 20 : 1(n − 9) and 22 : 1(n − 11) indicate intense lipid deposition, whereas these rates were low in females. The differences in lipid assimilation and turnover clearly show that the copepod species exhibit a high variability and plasticity to adapt their lipid production to their various life phases.This article is part of the XX ‘The next horizons for lipids as ‘trophic biomarkers': evidence and significance of consumer modification of dietary fatty acids'.