Deficiency of biodegradable plastic-degrading enzyme production in a gene-deletion mutant of phyllosphere yeast, Pseudozyma antarctica defective in mannosylerythritol lipid biosynthesis ...

• Main Authors: Saika, Azusa, Koike, Hideaki, Yarimizu, Tohru, Watanabe, Takashi, Kitamoto, Hiroko, Morita, Tomotake
• Format: Article in Journal/Newspaper
• Language: unknown
• Published: figshare 2019
• Subjects: Biochemistry; Microbiology; FOS Biological sciences; Genetics; Biological Sciences not elsewhere classified; Science Policy; Infectious Diseases; FOS Health sciences; Computational Biology; Antarc*; Antarctica; antarcticus
• Online Access: https://dx.doi.org/10.6084/m9.figshare.c.4567742; https://springernature.figshare.com/collections/Deficiency_of_biodegradable_plastic-degrading_enzyme_production_in_a_gene-deletion_mutant_of_phyllosphere_yeast_Pseudozyma_antarctica_defective_in_mannosylerythritol_lipid_biosynthesis/4567742

Abstract The basidiomycetous yeast Pseudozyma antarctica (currently designated Moesziomyces antarcticus) produces extracellular enzymes and glycolipids, including mannosylerythritol lipids (MELs), which are biosurfactants. Strain GB-4(0) of this species was previously isolated from rice husks and produces biodegradable plastic-degrading enzyme (Pseudozyma antarctica esterase; PaE). In this study, we generated a MEL biosynthesis-deficient strain (∆PaEMT1) by deleting the gene PaEMT1, which is essential to MEL biosynthesis in strain GB-4(0). The resulting ∆PaEMT1 strain showed deficient PaE activity, and the corresponding signal was hardly detected in its culture supernatant through western blotting analysis using rabbit anti-PaE serum. On the other hand, the relative expression of the gene PaCLE1, encoding PaE, was identical between GB-4(0) and ∆PaEMT1 based on quantitative real-time PCR. When strain ∆PaEMT1 was grown in culture media supplemented with various surfactants, i.e., Tween20, BRIJ35 and ...