Isolation and complete genome sequence of the thermophilic Geobacillus sp. 12AMOR1 from an Arctic deep-sea hydrothermal vent site

• Main Authors: Wissuwa, Juliane, Stokke, Runar, Anita-Elin Fedøy, Lian, Kjersti, Smalås, Arne, Steen, Ida
• Format: Article in Journal/Newspaper
• Language: unknown
• Published: Figshare 2016
• Subjects: Biochemistry; Microbiology; FOS Biological sciences; Genetics; Evolutionary Biology; 59999 Environmental Sciences not elsewhere classified; FOS Earth and related environmental sciences; 39999 Chemical Sciences not elsewhere classified; FOS Chemical sciences; Ecology; 20199 Astronomical and Space Sciences not elsewhere classified; FOS Physical sciences; 69999 Biological Sciences not elsewhere classified; Inorganic Chemistry; Arctic; Jan Mayen
• Online Access: https://dx.doi.org/10.6084/m9.figshare.c.3596975; https://figshare.com/collections/Isolation_and_complete_genome_sequence_of_the_thermophilic_Geobacillus_sp_12AMOR1_from_an_Arctic_deep-sea_hydrothermal_vent_site/3596975

Abstract Members of the genus Geobacillus have been isolated from a wide variety of habitats worldwide and are the subject for targeted enzyme utilization in various industrial applications. Here we report the isolation and complete genome sequence of the thermophilic starch-degrading Geobacillus sp. 12AMOR1. The strain 12AMOR1 was isolated from deep-sea hot sediment at the Jan Mayen hydrothermal Vent Site. Geobacillus sp. 12AMOR1 consists of a 3,410,035 bp circular chromosome and a 32,689 bp plasmid with a G + C content of 52 % and 47 %, respectively. The genome comprises 3323 protein-coding genes, 88 tRNA species and 10 rRNA operons. The isolate grows on a suite of sugars, complex polysaccharides and proteinous carbon sources. Accordingly, a versatility of genes encoding carbohydrate-active enzymes (CAZy) and peptidases were identified in the genome. Expression, purification and characterization of an enzyme of the glycoside hydrolase family 13 revealed a starch-degrading capacity and high thermal stability with a melting temperature of 76.4 °C. Altogether, the data obtained point to a new isolate from a marine hydrothermal vent with a large bioprospecting potential.