Steroidal composition and cytotoxic activity from fruiting body of Cortinarius xiphidipus

• Main Authors: Torres, Solange, Cajas, Daniel, Goetz Palfner, Allisson Astuya, Ambbar Aballay, Pérez, Claudia, Hernández, Víctor, Becerra, José
• Format: Article in Journal/Newspaper
• Language: unknown
• Published: Figshare 2016
• Subjects: Biochemistry; Medicine; Microbiology; FOS Biological sciences; Physiology; Pharmacology; Biotechnology; 59999 Environmental Sciences not elsewhere classified; FOS Earth and related environmental sciences; 39999 Chemical Sciences not elsewhere classified; FOS Chemical sciences; Ecology; 69999 Biological Sciences not elsewhere classified; Developmental Biology; Cancer; Antarctic; Magallanes; Antarc*; Antarctica
• Online Access: https://dx.doi.org/10.6084/m9.figshare.c.3256627; https://figshare.com/collections/Steroidal_composition_and_cytotoxic_activity_from_fruiting_body_of_i_Cortinarius_xiphidipus_i_/3256627

From the fruiting body of ectomycorrhizal fungi Cortinarius xiphidipus , sterols were identified from the crude extract and the cytotoxic effect of ergosta-4, 6, 8(14), 22-tetraen-3-one (ergone) was evaluated. Ten sterols including ergosta-3,5,7,9(11),22-pentaene, (22E)-ergosta-5,7,9(11),22-tetraen-3b-ol, (3β,22E)-ergosta-5,7,22-trien-3-ol, (22E)-ergosta-7,22-dien-3-ol, neoergosterol, (3β)-ergosta-5,8-dien-3-ol, (3β)-ergosta-7-en-3-ol, stigmasterol, stigmasterol 22,23-dihydro and (22E)-ergosta-4,6,8(14),22-tetraen-3-one were identified from the crude extract. The cytotoxic activity of the sterol fraction containing ergosta-4, 6, 8(14), 22-tetraen-3-one was assessed on four tumour cell lines (Neuro-2a, Saos-2, MCF7 and LNCaP-C42). The cytotoxic activity against the four tumour cell lines tested, being Neuro-2a and Saos-2 the most sensitive, with a half-maximal inhibitory concentration (IC 50 ) of 20.8 ± 2.2 and 27.8 ± 1.0 μg/mL, respectively. This is the first report of this Antarctic fungi collected in the Magallanes and Chilean Antarctica Region. This work represents a potential source for the development of anticancer drugs. '(22E)-ergosta-4,6,8(14),22-tetraen-3-one'