Steroidal composition and cytotoxic activity from fruiting body of Cortinarius xiphidipus

From the fruiting body of ectomycorrhizal fungi Cortinarius xiphidipus , sterols were identified from the crude extract and the cytotoxic effect of ergosta-4, 6, 8(14), 22-tetraen-3-one (ergone) was evaluated. Ten sterols including ergosta-3,5,7,9(11),22-pentaene, (22E)-ergosta-5,7,9(11),22-tetraen-...

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Bibliographic Details
Main Authors: Torres, Solange, Cajas, Daniel, Goetz Palfner, Allisson Astuya, Ambbar Aballay, Pérez, Claudia, Hernández, Víctor, Becerra, José
Format: Article in Journal/Newspaper
Language:unknown
Published: Figshare 2016
Subjects:
Biochemistry
Medicine
Microbiology
FOS Biological sciences
Physiology
Pharmacology
Biotechnology
59999 Environmental Sciences not elsewhere classified
FOS Earth and related environmental sciences
39999 Chemical Sciences not elsewhere classified
FOS Chemical sciences
Ecology
69999 Biological Sciences not elsewhere classified
Developmental Biology
Cancer
Antarctic
Magallanes
Antarc*
Antarctica
Online Access:https://dx.doi.org/10.6084/m9.figshare.c.3256627
https://figshare.com/collections/Steroidal_composition_and_cytotoxic_activity_from_fruiting_body_of_i_Cortinarius_xiphidipus_i_/3256627
Description
Summary:From the fruiting body of ectomycorrhizal fungi Cortinarius xiphidipus , sterols were identified from the crude extract and the cytotoxic effect of ergosta-4, 6, 8(14), 22-tetraen-3-one (ergone) was evaluated. Ten sterols including ergosta-3,5,7,9(11),22-pentaene, (22E)-ergosta-5,7,9(11),22-tetraen-3b-ol, (3β,22E)-ergosta-5,7,22-trien-3-ol, (22E)-ergosta-7,22-dien-3-ol, neoergosterol, (3β)-ergosta-5,8-dien-3-ol, (3β)-ergosta-7-en-3-ol, stigmasterol, stigmasterol 22,23-dihydro and (22E)-ergosta-4,6,8(14),22-tetraen-3-one were identified from the crude extract. The cytotoxic activity of the sterol fraction containing ergosta-4, 6, 8(14), 22-tetraen-3-one was assessed on four tumour cell lines (Neuro-2a, Saos-2, MCF7 and LNCaP-C42). The cytotoxic activity against the four tumour cell lines tested, being Neuro-2a and Saos-2 the most sensitive, with a half-maximal inhibitory concentration (IC 50 ) of 20.8 ± 2.2 and 27.8 ± 1.0 μg/mL, respectively. This is the first report of this Antarctic fungi collected in the Magallanes and Chilean Antarctica Region. This work represents a potential source for the development of anticancer drugs. '(22E)-ergosta-4,6,8(14),22-tetraen-3-one'

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