Steroidal composition and cytotoxic activity from fruiting body of Cortinarius xiphidipus
From the fruiting body of ectomycorrhizal fungi Cortinarius xiphidipus , sterols were identified from the crude extract and the cytotoxic effect of ergosta-4, 6, 8(14), 22-tetraen-3-one (ergone) was evaluated. Ten sterols including ergosta-3,5,7,9(11),22-pentaene, (22E)-ergosta-5,7,9(11),22-tetraen-...
| Main Authors: | , , , , , , , |
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| Format: | Article in Journal/Newspaper |
| Language: | unknown |
| Published: |
Figshare
2016
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| Subjects: | |
| Online Access: | https://dx.doi.org/10.6084/m9.figshare.c.3256627.v1 https://figshare.com/collections/Steroidal_composition_and_cytotoxic_activity_from_fruiting_body_of_i_Cortinarius_xiphidipus_i_/3256627/1 |
| Summary: | From the fruiting body of ectomycorrhizal fungi Cortinarius xiphidipus , sterols were identified from the crude extract and the cytotoxic effect of ergosta-4, 6, 8(14), 22-tetraen-3-one (ergone) was evaluated. Ten sterols including ergosta-3,5,7,9(11),22-pentaene, (22E)-ergosta-5,7,9(11),22-tetraen-3b-ol, (3β,22E)-ergosta-5,7,22-trien-3-ol, (22E)-ergosta-7,22-dien-3-ol, neoergosterol, (3β)-ergosta-5,8-dien-3-ol, (3β)-ergosta-7-en-3-ol, stigmasterol, stigmasterol 22,23-dihydro and (22E)-ergosta-4,6,8(14),22-tetraen-3-one were identified from the crude extract. The cytotoxic activity of the sterol fraction containing ergosta-4, 6, 8(14), 22-tetraen-3-one was assessed on four tumour cell lines (Neuro-2a, Saos-2, MCF7 and LNCaP-C42). The cytotoxic activity against the four tumour cell lines tested, being Neuro-2a and Saos-2 the most sensitive, with a half-maximal inhibitory concentration (IC 50 ) of 20.8 ± 2.2 and 27.8 ± 1.0 μg/mL, respectively. This is the first report of this Antarctic fungi collected in the Magallanes and Chilean Antarctica Region. This work represents a potential source for the development of anticancer drugs. '(22E)-ergosta-4,6,8(14),22-tetraen-3-one' |
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