Additional file 1 of Rapid and simple colorimetric detection of multiple influenza viruses infecting humans using a reverse transcriptional loop-mediated isothermal amplification (RT-LAMP) diagnostic platform

: Figure S1. Specificity of RT-LAMP. The specificity of the RT-LAMP assay was tested using (A) individual and (B) mixed influenza virus samples listed below. RT-LAMP amplicon was confirmed using 2% agarose gel electrophoresis. The positive sample (yellow color) has a typical ladder-like pattern of R...

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Bibliographic Details
Main Authors: Ahn, Su, Baek, Yun, Khristine Lloren, Won-Suk Choi, Jeong, Ju, Khristine Antigua, Hyeok-Il Kwon, Park, Su-Jin, Eun-Ha Kim, Kim, Young-Il, Si, Young-Jae, Hong, Seung, Shin, Kyeong, Sungkun Chun, Choi, Young, Song, Min-Suk
Format: Text
Language:unknown
Published: figshare 2019
Subjects:
Medicine
Cell Biology
Genetics
FOS Biological sciences
Molecular Biology
Neuroscience
Biotechnology
39999 Chemical Sciences not elsewhere classified
FOS Chemical sciences
Immunology
FOS Clinical medicine
Cancer
Hematology
110309 Infectious Diseases
FOS Health sciences
Plant Biology
60506 Virology
Brisbane
gyrfalcon
Online Access:https://dx.doi.org/10.6084/m9.figshare.9210812.v1
https://springernature.figshare.com/articles/Additional_file_1_of_Rapid_and_simple_colorimetric_detection_of_multiple_influenza_viruses_infecting_humans_using_a_reverse_transcriptional_loop-mediated_isothermal_amplification_RT-LAMP_diagnostic_platform/9210812/1
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Summary:: Figure S1. Specificity of RT-LAMP. The specificity of the RT-LAMP assay was tested using (A) individual and (B) mixed influenza virus samples listed below. RT-LAMP amplicon was confirmed using 2% agarose gel electrophoresis. The positive sample (yellow color) has a typical ladder-like pattern of RT-LAMP reaction. (a) B-Vic:B/Brisbane/60/2008 (Victoria lineage); (b) B-Yam: B/Phuket/3073/2013 (Yamagata lineage); (c) H1N1:A/California/04/2009; (d) H3N2: A/Perth/16/2009; (e) aH5N1:A/Em/Korea/w149/2006; (f) hH5N6 vac: A/Sichuan/26221/2014; (g) aH5N8:A/Em/Korea/w468/2014; (h) aH5N8 vac:A/gyrfalcon/Washington/41088â 6/2014; (i) hH7N9:A/Anhui/1/2013; N.C.: Negative control (D.W.). Figure S2. Sensitivity of the RT-LAMP assay compared with conventional methods. To estimate the sensitivity of the RT-LAMP assay, RNA samples from each influenza viruses were 10-fold serially diluted and used as templates for the RT-LAMP assay (A), conventional RT-PCR (B), and real-time qRT-PCR (C). RT-LAMP results are visualized colorimetrically and by gel-electrophoresis. Results of conventional RT-PCR (B) and real-time qRT-PCR (C) are visualized using gel electrophoresis and cycle threshold (Ct) values, respectively. Please see Table 2 for the full name of virus used. N.C., negative control. Table S1. Primers for detection of influenza viruses of other subtypes and human 3 respiratory disease viruses. (PDF 565 kb)

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