Remarkable stability of Candida antarctica lipase B immobilized via cross-linking aggregates (CLEA) in deep eutectic solvents

The combination of Deep-eutectic-solvents (DES) with water as “co-solvent” enables a low-viscous reaction medium that keeps its “non-conventional” nature and thus enables synthetic lyophilization reactions (e.g. esterification) catalyzed by hydrolases. Substrates with different polarity may be emplo...

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Bibliographic Details
Main Authors: Guajardo, Nadia, Ahumada, Katherine, María, Pablo Domínguez De, Schrebler, Rodrigo A.
Format: Text
Language:unknown
Published: Taylor & Francis 2019
Subjects:
Biochemistry
Biotechnology
39999 Chemical Sciences not elsewhere classified
FOS Chemical sciences
Sociology
FOS Sociology
Inorganic Chemistry
110309 Infectious Diseases
FOS Health sciences
Antarc*
Antarctica
Online Access:https://dx.doi.org/10.6084/m9.figshare.7808444.v1
https://tandf.figshare.com/articles/Remarkable_stability_of_i_Candida_antarctica_i_lipase_B_immobilized_i_via_i_cross-linking_aggregates_CLEA_in_deep_eutectic_solvents/7808444/1
Description
Summary:The combination of Deep-eutectic-solvents (DES) with water as “co-solvent” enables a low-viscous reaction medium that keeps its “non-conventional” nature and thus enables synthetic lyophilization reactions (e.g. esterification) catalyzed by hydrolases. Substrates with different polarity may be employed. This paper shows how the enzyme immobilization with cross-linking aggregates (CLEA) leads to highly stable and active immobilized catalysts in different DES. As a remarkable case, when choline chloride-glycerol DES is used, CLEA derivatives of Candida antarctica lipase B (CLEA-CALB) are stable for at least 14 days without any loss of activity. The immobilized biocatalysts are applied in non-viscous DES-water blends (8% v/v) to catalyze the esterification of benzoic acid and glycerol to furnish glyceryl monobenzoate (α-MBG) in productivities of ∼35 g α-MBG L −1 d −1 . Compared to other commercial immobilized CALB, the CLEA-CALB derivatives rendered more product (higher conversions by 30%). Moreover, CLEA derivatives were successfully reused for six times without any loss of activity. Given the ease of immobilization (CLEA), their excellent performance in DES and the low viscosity of the DES-water blends, the reported approach may be useful for many synthetic procedures and even for continuous processes with largely optimized outcomes.

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