Additional file 2: of Nervous system development in the Pacific oyster, Crassostrea gigas (Mollusca: Bivalvia)
Figure S2. Specificity of ChAT antibodies in adult tissues of Crassostrea gigas and expression of ChAT-ir in nervous elements. a: Western blot of total protein lysates from adult oyster tissue probes stained with goat polyclonal antibodies against rat ChAT. No specific band is detected in the adult...
| Main Authors: | , , , |
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| Format: | Still Image |
| Language: | unknown |
| Published: |
figshare
2018
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| Subjects: | |
| Online Access: | https://dx.doi.org/10.6084/m9.figshare.6132404.v1 https://springernature.figshare.com/articles/Additional_file_2_of_Nervous_system_development_in_the_Pacific_oyster_Crassostrea_gigas_Mollusca_Bivalvia_/6132404/1 |
| Summary: | Figure S2. Specificity of ChAT antibodies in adult tissues of Crassostrea gigas and expression of ChAT-ir in nervous elements. a: Western blot of total protein lysates from adult oyster tissue probes stained with goat polyclonal antibodies against rat ChAT. No specific band is detected in the adult oyster tissues. The positive signal only corresponds to a protein band with a molecular weight of 69 kDa in the cell lysate of mouse spinal cord. b: Immunostaining of frozen sections of adult oyster tissues with ChAT/TUBULIN antibodies show the absence of a positive ChAT-ir signal in all tested tissues. c: Confocal image of the 7-dpf veliger stained with ChAT/TUBULIN antibodies shows no positive inner structures. The signal observed along the shell edge and in the center of the larval body is likely non-specific fluorescence. Scale bar = 100 μm in b and 50 μm in c. (TIFF 2992 kb) |
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