Isotryptophan from Antarctic Bacillus amyloliquefaciens Pc3: purification, identification, characterization, and antifungal activity

• Main Authors: Pengfei Cui, Wenbin Guo, Xinhua Chen
• Format: Text
• Language: unknown
• Published: Taylor & Francis 2017
• Subjects: Biochemistry; Microbiology; FOS Biological sciences; Pharmacology; Biotechnology; 59999 Environmental Sciences not elsewhere classified; FOS Earth and related environmental sciences; 39999 Chemical Sciences not elsewhere classified; FOS Chemical sciences; Ecology; 110309 Infectious Diseases; FOS Health sciences; Plant Biology; 60506 Virology; Antarctic; The Antarctic; Antarc*
• Online Access: https://dx.doi.org/10.6084/m9.figshare.4509182.v1; https://tandf.figshare.com/articles/journal_contribution/Isotryptophan_from_Antarctic_i_Bacillus_amyloliquefaciens_i_Pc3_purification_identification_characterization_and_antifungal_activity/4509182/1

Strain Pc3 was isolated from Antarctic seawater and identified as Bacillus amyloliquefaciens . A compound with antifungal activity was purified from the fermentation supernatant of B. amyloliquefaciens Pc3. Its structure was determined to be (S)-2-amino-3-(1H-indol-2-yl) propanoic acid, named as isotryptophan, based on detailed analysis of its nuclear magnetic resonance (NMR) and high-resolution electrospray ionisation mass spectrometry (HR–ESI–MS). Isotryptophan exhibited good thermal stability and antifungal activity against several plant-pathogenic fungi with low minimum inhibitory concentrations. Therefore, the characterisation of isotryptophan from the Antarctic B. amyloliquefaciens Pc3 will facilitate its potential application in the control of plant-pathogenic fungal infection.