Isotryptophan from Antarctic Bacillus amyloliquefaciens Pc3: purification, identification, characterization, and antifungal activity

Strain Pc3 was isolated from Antarctic seawater and identified as Bacillus amyloliquefaciens . A compound with antifungal activity was purified from the fermentation supernatant of B. amyloliquefaciens Pc3. Its structure was determined to be (S)-2-amino-3-(1H-indol-2-yl) propanoic acid, named as iso...

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Bibliographic Details
Main Authors: Pengfei Cui, Wenbin Guo, Xinhua Chen
Format: Text
Language:unknown
Published: Taylor & Francis 2017
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Online Access:https://dx.doi.org/10.6084/m9.figshare.4509182.v1
https://tandf.figshare.com/articles/journal_contribution/Isotryptophan_from_Antarctic_i_Bacillus_amyloliquefaciens_i_Pc3_purification_identification_characterization_and_antifungal_activity/4509182/1
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Summary:Strain Pc3 was isolated from Antarctic seawater and identified as Bacillus amyloliquefaciens . A compound with antifungal activity was purified from the fermentation supernatant of B. amyloliquefaciens Pc3. Its structure was determined to be (S)-2-amino-3-(1H-indol-2-yl) propanoic acid, named as isotryptophan, based on detailed analysis of its nuclear magnetic resonance (NMR) and high-resolution electrospray ionisation mass spectrometry (HR–ESI–MS). Isotryptophan exhibited good thermal stability and antifungal activity against several plant-pathogenic fungi with low minimum inhibitory concentrations. Therefore, the characterisation of isotryptophan from the Antarctic B. amyloliquefaciens Pc3 will facilitate its potential application in the control of plant-pathogenic fungal infection.