Immobilization of Candida antarctica lipase B (CALB) on surface-modified rice husk ashes (RHA) via physical adsorption and cross-linking methods
In the present study, the recovery of activity of Candida antarctica lipase B (CALB) immobilized onto surface-modified rice husk ash (RHA) was 90% for both cross-linking and adsorption methods. Both cross-linked and adsorbed immobilized preparations were very stable, retaining more than 48% of their...
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| Format: | Text |
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Taylor & Francis
2016
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| Online Access: | https://dx.doi.org/10.6084/m9.figshare.4244546.v1 https://tandf.figshare.com/articles/journal_contribution/Immobilization_of_i_Candida_antarctica_i_lipase_B_CALB_on_surface-modified_rice_husk_ashes_RHA_via_physical_adsorption_and_cross-linking_methods/4244546/1 |
| Summary: | In the present study, the recovery of activity of Candida antarctica lipase B (CALB) immobilized onto surface-modified rice husk ash (RHA) was 90% for both cross-linking and adsorption methods. Both cross-linked and adsorbed immobilized preparations were very stable, retaining more than 48% of their activity over the range of temperatures studied. The optimum temperature and optimum pH values were 37 °C and 7.0, respectively for both immobilized preparations, while the relative activities after storage at 4.0 °C for 60 days were 55% and 65% using cross-linking and adsorption methods, respectively. Also, the activity of the immobilized lipase began to decrease after 10 cycles, more than 58% of the initial activities were still retained after 10 cycles for both immobilization methods. These results indicated that lipase immobilized by cross-linking and adsorption not only effected activity recovery, but also remarkably effected stability, reusability and application adaptability. It can be concluded that, surface-modified RHA can be used as alternative supports for immobilization of CALB for polymerization reactions. |
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